Risk of bias assessment of post-stroke mortality machine learning predictive models: Systematic review.

Background: Stroke is a major cause of mortality and permanent disability worldwide. Precise prediction of post-stroke mortality is essential for guiding treatment decisions and rehabilitation planning. The ability of Machine learning models to process large amounts of data, offer a promising alternative for improving mortality prediction in stroke patients.

Health-Related Quality of Life in Breast Cancer Patients during Chemotherapy: A Cross-Sectional Study Using the EORTC QLQ-C30 and BR45.

Objective: To assess health-related quality of life (HRQoL) using the European Organisation for Research and Treatment of Cancer Quality of Life Questionnaire for Breast Cancer (EORTC QLQ-BR45) in conjunction with the Core questionnaire (EORTC QLQ-C30) in breast cancer patients receiving chemotherapy.

Materials And Methods: This prospective, cross-sectional study was conducted in the oncology department of a tertiary care hospital for six months. Patients aged ≥18 years, diagnosed with breast cancer, and who had received at least three chemotherapy cycles were included in the study.

5-Fluorouracil- and Sesamol-Loaded Transliposomal Gel for Skin Cancer: , and Dermatokinetic Evaluation.

This study explores a novel approach to managing skin conditions through a combination therapy utilizing a phospholipid-enriched edge activator-based nanoformulation. 5-Fluorouracil (5-FU)- and sesamol (SES)-loaded transliposomes (FS-TL) were developed using a thin film hydration method and optimized using Box-Behnken Design. FS-TL characterization indicated a vesicle size of 165.

Transcriptomic insights on impaired survival and enhanced pesticide susceptibility following knockdown of Syntaxin5 in Locusta migratoria.

Membrane fusion, essential for a variety of biological processes, is ubiquitous and critical in all living organisms. The soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) comprise the core machinery that mediates membrane fusion. Syntaxin5 (Stx5) in mammals and its ortholog Sed5p in yeast are SNARE proteins that have been reported to mediate anterograde and retrograde endoplasmic reticulum (ER)-Golgi trafficking.

Comparative evaluation of management practices among large-scale broiler farms of Punjab, Pakistan.

Limited research is available on the management practices of commercial broiler chicken production in Pakistan. Notably, the rapid expansion of environmentally controlled broiler farms in Punjab in recent years remains underexplored, with scarce information on this growing enterprise. The current study compared the management practices at 100 commercial broiler farms in Punjab, Pakistan, among different regions.

Heavy metal contamination in some commercial marine fishes of Pakistan.

Anthropogenic activities, such as industrial discharge, cargo handling, and untreated wastewater release, have led to significant heavy metal contamination along the Karachi coastline, posing risks to marine life and human health. Current study evaluated the health risks associated with consuming five fish species (, , , , and ) by analysing muscle tissues for arsenic, chromium, copper, and nickel accumulation. and exhibited hazard index (HI) values greater than 1, indicating potential non-carcinogenic risks to adults, particularly due to high arsenic levels.

Loss of mitochondrial amidoxime-reducing component 1 (mARC1) prevents disease progression by reducing fibrosis in multiple mouse models of chronic liver disease.

Background: Metabolic dysfunction-associated steatotic liver disease is a prevalent disease that affects nearly one-third of the global population. Recent genome-wide association studies revealed that a common missense variant in the gene encoding mitochondrial amidoxime reducing component 1 (mARC1) is associated with protection from metabolic dysfunction-associated steatotic liver disease, all-cause cirrhosis, and liver-related mortality suggesting a role for mARC1 in liver pathophysiology; however, little is known about its function in the liver. In this study, we aimed to evaluate the impact of mARC1 hepatoprotective variants on protein function, the effect of loss of mARC1 on cellular lipotoxic stress response, and the effect of global or hepatocyte-specific loss of mARC1 in various mouse models of metabolic dysfunction-associated steatohepatitis and liver fibrosis.

Harnessing the potential of chloroplast-derived expression elements for enhanced production of cellulases in .

Thermophilic cellulases can play a crucial part in the efficient breakdown of cellulose-a major component of lignocellulosic plant biomass, however, their commercial production needs simple and robust biomanufacturing biosystems. In this study, two cellulases (β-glucosidase and endoglucanase) were heterologously expressed in under a chloroplast-derived constitutive promoter and expression-enhancing terminator. The genes encoding the cellulases were sourced from a thermophilic bacterium to exploit their industrially needed thermotolerance potential.

Impact of temperatures on malaria incidence in vulnerable regions of Pakistan: empirical evidence and future projections.

Malaria remains a major health challenge in developing countries, with climate change intensifying its impact. Pakistan is among the most vulnerable nations. This study examines the relationship between temperature and malaria cases in two highly affected districts, Bannu and Lakki Marwat, to inform climate-adaptive interventions.

Innovative approaches to eczema treatment: A review of Fevipiprant and its potential as a new therapeutic agent.

Eczema is also known as atopic dermatitis, which goes on to affect the skin as a chronic inflammatory disease. It is associated with a constant feeling of scratchiness, erthyma and disruption of the natural skin barrier. Treatment provided at present may improve some of the symptoms, for instance use of corticosteroids or immunosuppressive agents, however, there is an overwhelming need for better focused and effective methods of treatment with minimal adverse effects.

Host-microbiome determinants of ready-to-use supplemental food efficacy in acute childhood malnutrition.

Ready-to-use supplemental foods (RUSF) are energy-dense meals formulated to prevent and treat moderate and severe childhood acute malnutrition (MAM and SAM) in high-risk settings. Although lifesaving, the degree and durability of weight recovery with RUSF is unpredictable. We examined whether environmental enteric dysfunction (EED) and gut microbiota perturbations are risk factors for RUSF failure in a birth cohort of 416 rural Pakistani children followed for growth, common childhood illnesses, and biomarkers from blood, urine, and stool.

Double extreme-cum-median ranked set sampling.

Extreme-cum-median ranked set sampling has been developed to address the problem of heterogeneity and outliers / extreme values. Double ranked set sampling has been suggested to obtain more reliable samples using the concept of degree of distinguishability. Dealing with heterogeneous and non-normal populations seems to be an area with a dearth of research.

Enhanced anticancer and biological activities of environmentally friendly Ni/Cu-ZnO solid solution nanoparticles.

The study investigates the impact of incorporating Ni and Cu into the lattice of ZnO nanoparticles (NPs) to enhance their anticancer and antioxidant properties. Characterization techniques including pXRD, FTIR, UV-visible absorption spectroscopy, FESEM, and EDAX confirm the successful synthesis and structural modifications of Ni/Cu-ZnO NPs. Anticancer activity against breast cancer (MDA) and normal skin (BHK-21) cells reveals dose-dependent cytotoxicity, with Ni/Cu-ZnO NPs exhibiting higher efficacy against MDA cells while being less harmful to BHK-21 cells.

Naringenin loaded solid lipid nanoparticles alleviate oxidative stress and enhance oral bioavailability of naringenin.

Naringenin (Nrg) is the most abundant natural dietary flavonoid with promising anti-inflammatory potential. However, its therapeutic application is limited due to poor absorption, low bioavailability, and inability to cross physiological barriers. Herein, we designed biocompatible solid lipid nanoparticles (SLNs) to overcome these challenges and to enhance the oral bioavailability and therapeutic potential of Nrg.

Beeswax-based nanoconstructs enriched dual responsive hydrogel for diabetic foot ulcers in streptozotocin-induced diabetic rats.

Diabetic foot ulcer (DFU) is a complicated pathophysiological process, and there is now no recognized treatment. Hyperglycemia, neuropathy, impaired angiogenesis, reactive oxygen species, and advanced glycation end products construct the distinctive wound environment of diabetic wounds. This study aimed to develop naringenin-ferulic acid beeswax-based nanoconstructs enriched dual-responsive hydrogel (NAR-FA NLC HG) for topical application for DFU.

Moisture Sorption by Low-Cost Pyridinium-Based Protic Ionic Liquids: Kinetics and Physico-Electrochemical Properties.

We report the synthesis of two pyridinium-based room temperature protic ionic liquids (PILs), pyridinium bisulfate, [HPyr][HSO] and pyridinium sulphate, [HPyr][SO] and investigation of the kinetics of their water sorption behaviour and its influence on their density, ionic conductivity, and potential windows. The PILs were synthesized by the reaction of pyridine base with an acid, HSO, under solventless conditions, and confirmed by FTIR spectroscopy and 1H NMR spectra. The appearance vibration bands in the 3095-3252 cm range for -NH stretching in the FTIR spectra and a peak at a chemical shift of 8.

Cultural Competency in Dental Practice: Navigating the Experiences and Perspectives of Dentists in South Punjab, Pakistan.

Introduction Cultural competence in healthcare embraces the principles of equal access and nondiscriminatory services in healthcare delivery. Dentists as significant healthcare professionals should definitely have the skills needed to diagnose and treat patients' conditions, but it is crucial to also address nontechnical skills such as cross-cultural communication, the ability to empathize, and inclusive-mindedness. Globally, cultural competency training has been included in healthcare educational programs; however, national practices reportedly have revolved around patients' clinical competencies and thus entailed overlooking the cultural dimensions of dental education that include diverse sociocultural factors that influence how dental practices are taught, learned, and delivered.

Comparative Outcomes of Transcatheter Versus Surgical Aortic Valve Replacement in Moderate-Risk Patients With Aortic Stenosis: A Systematic Review of Clinical Trials.

Aortic stenosis (AS) is a prevalent condition among the elderly, characterized by the narrowing of the aortic valve, which, if untreated, can lead to heart failure and decreased quality of life in terms of reduced activity and high mortality in one to two years. Surgical aortic valve replacement (SAVR) has long been the standard treatment for AS. However, it poses significant risks, particularly in older patients with comorbidities.

Navigating workplace conflicts and fostering innovative behaviors: the role of job commitment and socio-instrumental ESM utilization.

This study explores the dynamics of intersection between workplace conflicts, job commitment, and socio-instrumental enterprise social media (ESM) utilization for improving innovation. This study proposes job commitment as a pivotal mediator that influences employees' attitudinal responses to the array of conflicts encountered in the workplace. Additionally, it introduce socio-instrumental ESM use as a potent moderator, influencing employees' appraisals of workplace conflicts.

The effects of tumor-derived exosomes enriched with miRNA-211a on B16F10 cells.

Introduction: Exosomes have emerged as a novel alternative delivery system for transporting small molecules. Tumor-derived exosomes (TEXs) possess anti-cancer properties and serve as natural carriers of microRNAs. Using this knowledge as a foundation, the current study evaluated the efficacy of delivering a miR-211 mimic B16F10 cell-derived exosomes to block the growth and development of a melanoma cell line.

Kinetics and thermodynamics investigations of efficient and eco-friendly removal of alizarin red S from water via acid-activated leaf powder and its magnetic iron oxide nanocomposite.

The present work aimed to highlight an efficient, readily accessible, and cost-effective adsorbent derived from (DS) leaf powder for removing the environmentally hazardous dye "alizarin red S" (ARS) from hydrous medium. A variant of the adsorbent is activated via sulfuric acid and composited with magnetic iron oxide nanoparticles (DSMNC). Both adsorbents are thoroughly characterized using techniques such as Fourier transform infrared spectroscopy, point of zero charge, energy-dispersive X-ray spectroscopy, and scanning electron microscopy, which show that they have a porous structure rich in active sites.

Cardiac prehabilitation, rehabilitation and education in first-time atrial fibrillation (AF) ablation (CREED AF): Study protocol for a randomised controlled trial.

Background: Atrial fibrillation (AF) is associated with significant morbidity/mortality. AF-ablation is an increasingly used treatment. Currently, first-time AF-ablation success is 40-80% at 1-year, depending on individual factors.