Influence of spacer length on the agglutination of glycolipid-incorporated liposomes by ConA as model membrane.

Through a systematic investigation of the agglutination of long chain mannolipid and glucolipid incorporated liposomes by concanavalin A (ConA) it was found that the agglutination was dependent on different factors. The studied factors reported here are (1) spacer length and (2) ground lipid matrix. The threshold and the relative saturating ConA binding concentration (saturation point to attain the binding saturation condition) of glycosides with varying spacer length for agglutination are dependent on the spacer length of the glycolipid.

Influence of spacer length on interaction of mannosylated liposomes with human phagocytic cells.

Purpose: To improve target specificity and uptake of liposomes by macrophages, one can improve high-affinity receptor binding to mannose determinants with their 175-kDa mannose receptor (MR), which is mainly influenced by the length and flexibility of the spacer between the carbohydrate head group and liposome surface. Liposomes containing alkylmannosides with hydrophilic spacers 0 to 8 ethyleneoxy units (EO) long (Man0..

Catalytic effects of galactose oxidase on micelle-forming galactolipids.

Catalytic effects of galactose oxidase on the oxidation of beta-D-galactose-carrying lipids with an oligo-ethylene glycol spacer (number of ethylene glycol units (n)=1, 2, 3, 6, 9, 13, and 20) were examined. The affinity of galactose oxidase for the galactose residue in the amphiphile (estimated by the inverse of the Michaelis constant, K(m)) was much higher than those for free D-galactose and small beta-D-galactopyranosides, and dependent on the length of the ethylene glycol spacer. That is, both below and above the critical micellar concentration, the 1/K(m) values decreased with an increase in the n value.