Zinc-based fixative as a novel approach for histological preservation: A comparative study with formalin-based fixatives.

Background: Formalin is a widely used histological fixative despite its carcinogenic properties, inadequate nucleic acid preservation, and prolonged fixation time.

Aim: The study intended to prepare a novel zinc-based fixative that is, formalin-free, and cost-effective, provides optimal preservation, with rapid penetration rates, and enhanced Hematoxylin/Eosin-stained sections.

Methods: Liver, kidney, brain, and small intestine specimens were collected from 10 adult laboratory rats () and preserved in formalin 4%, formalin 10%, neutral buffered formalin (NBF), and the experimental EZAT solution.

Morphological, histological, and histochemical study of the adult golden hamster () spleen.

Background: The golden hamster is a choice model for investigating many visceral and splenic infections and neoplastic and retrospective lesions.

Aim: To study hamsters' spleen's morphological, histological, and histochemical structure.

Methods: Samples were collected from eight healthy adult golden hamsters and then fixed with 10% buffered formalin.