Analyte stability during the total testing process: studies of vitamins A, D and E by LC-MS/MS.

Background: There are limited evidence based studies demonstrating the stability of fat-soluble vitamins (FSV) measured in blood. This study aimed to examine the effects of light, temperature and time on vitamins A, D and E throughout the total testing process.

Methods: Four experiments were conducted.

Fat-Soluble Vitamins: Clinical Indications and Current Challenges for Chromatographic Measurement.

Fat-soluble vitamins, including vitamins A, D and E, are required for a wide variety of physiological functions. Over the past two decades, deficiencies of these vitamins have been associated with increased risk of cancer, type II diabetes mellitus and a number of immune system disorders. In addition, there is increasing evidence of interactions between these vitamins, especially between vitamins A and D.

A simultaneous quantitative method for vitamins A, D and E in human serum using liquid chromatography-tandem mass spectrometry.

Non-classical roles of fat-soluble vitamins (FSVs) in many pathologies including cancer have been identified. There is also evidence of hormonal interactions between two of these vitamins, A and D. As a result of this enhanced clinical association with disease, translational clinical research and laboratory requests for FSV measurement has significantly increased.

Comparison of three commercial calibrators for alpha-tocopherol using liquid chromatography-tandem mass spectrometry.

Objectives: Alpha-tocopherol is the predominant form of vitamin E in plasma and is routinely measured to assess vitamin E status. Agreement between vitamin E assays is essential to provide consistent result interpretation. Lack of agreement among calibrators is potentially a significant obstacle to method harmonization.