Publications by authors named "Alfred Lechner"
Anal Bioanal Chem
September 2015
Article Synopsis
- Large arrays of tiny chambers (femtoliter-sized) are crucial for studying single molecules and bioanalytical tasks, which have been optimized using two methods: etching fused silica slides and molding with PDMS.*
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- The study focused on observing the enzyme β-galactosidase in these chambers, employing wide-field fluorescence microscopy to monitor reactions while preventing diffusion of products through a new sealing method.*
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- This optimized system allows for a three-function enzyme assay: counting active enzymes, averaging turnover rates without concentration knowledge, and analyzing the variability in enzyme activity, revealing important insights into enzyme conformational differences.*
