Publications by authors named "Alexis Rae Del Castillo"
Article Synopsis
- Neutron diffraction is more effective than X-ray diffraction for pinpointing hydrogen (H) atom locations in biological structures, especially in proteins like Pyrococcus furiosus rubredoxin.
- The study compares high-resolution neutron data (1.65 A) and X-ray data (1.1 A) to demonstrate that neutron data are about eight times more reliable for determining positions of deuterium (D) atoms than X-ray data at various temperatures.
- Joint analysis of both X-ray and neutron data significantly improves the accuracy of identifying D atom positions, identifying approximately 90% of positions for D-rubredoxin and 80% for HD-rubredoxin, compared to very low percentages with X-ray data alone.
Characterization of sol-gel-encapsulated proteins using small-angle neutron scattering.
ACS Appl Mater Interfaces
October 2009
Entrapment of biomolecules in silica-derived sol-gels has grown into a vibrant area of research since it was originally demonstrated. However, accessing the consequences of entrapment on biomolecules and the gel structure remains a major challenge in characterizing these biohybrid materials. We present the first demonstration that it is possible with small-angle neutron scattering (SANS) to study the conformation of dilute proteins that are entrapped in transparent and dense sol-gels.
View Article and Find Full Text PDF