Publications by authors named "Alexey M Afonin"

Respiratory viruses have a significant impact on health, as highlighted by the COVID-19 pandemic. Exposure to air pollution can contribute to viral susceptibility and be associated with severe outcomes, as suggested by recent epidemiological studies. Furthermore, exposure to particulate matter (PM), an important constituent of air pollution, is linked to adverse effects on the brain, including cognitive decline and Alzheimer's disease (AD).

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Global emphasis on enhancing prevention and treatment strategies necessitates an increased understanding of the biological mechanisms of psychopathology. Plasma proteomics is a powerful tool that has been applied in the context of specific mental disorders for biomarker identification. The p-factor, also known as the "general psychopathology factor", is a concept in psychopathology suggesting that there is a common underlying factor that contributes to the development of various forms of mental disorders.

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The study is aimed at revealing the effects of inoculation on the transcriptome of leaves at the early (second leaf formation) and later (flowering) stages of plant development. A pot experiment was conducted under conditions of low phosphorus (P) level in the substrate. plants were characterized by high mycorrhizal growth response and mycorrhization parameters.

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Soil phage AP-16-3, a strain phylogenetically close to phage 16-3, was isolated in a mountainous region of Dagestan, belonging to the origin of cultivated plants in the Caucasus, according to Vavilov N.I. The genome of phage AP-16-3 is 61 kbp in size and contains 62 ORFs, of which 42 ORFs have homologues in the genome of phage 16-3, which was studied in the 1960s-1980s.

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Article Synopsis
  • The study investigates how microorganisms decompose oat straw in soil over six months, highlighting changes in microbial communities during this process.
  • During straw decomposition, three distinct phases of microbial activity and diversity were observed: an active early phase, a low-activity middle phase, and a high-diversity late phase.
  • Key findings include the identification of major bacterial and fungal groups that play a role in cellulose degradation, with significant genetic evidence of their capability to break down organic materials.
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Various legume plants form root nodules in which symbiotic bacteria (rhizobia) fix atmospheric nitrogen after differentiation into a symbiotic form named bacteroids. In some legume species, bacteroid differentiation is promoted by defensin-like nodule-specific cysteine-rich (NCR) peptides. NCR peptides have best been studied in the model legume Gaertn.

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Recycling plant matter is one of the challenges facing humanity today and depends on efficient lignocellulose degradation. Although many bacterial strains from natural substrates demonstrate cellulolytic activities, the CAZymes (Carbohydrate-Active enZYmes) responsible for these activities are very diverse and usually distributed among different bacteria in one habitat. Thus, using microbial consortia can be a solution to rapid and effective decomposition of plant biomass.

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Rhizobium ruizarguesonis (Rhizobium leguminosarum) strain 1TK341 was isolated from pink nodules of fixation-negative mutant line P61 of pea (Pisum sativum L.) grown in soil. Here, we report the draft genome sequence of the strain.

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Sinorhizobium meliloti is a symbiotic bacterial species forming nitrogen-fixing nodules on roots of annual and perennial spp. We report the full genome sequence of S. meliloti strain AK76, an effective symbiont of the wild diploid plant Medicago lupulina grown in the Mugodgary Mountain region, Kazakhstan.

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() is a common name for several genospecies of rhizobia able to form nitrogen-fixing nodules on the roots of pea ( L.) while undergoing terminal differentiation into a symbiotic form called bacteroids. In this work, we used Oxford Nanopore sequencing to analyze the genome methylation states of the free-living and differentiated forms of the strain RCAM1026.

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The genome of a symbiotically effective salt-tolerant strain, S35m, isolated from alfalfa rhizosphere in soil native to the Caucasus region, was sequenced. Genomic islands, prophages, and elements of a potential CRISPR/Cas I type (Cas3_0_I) system were identified in the genome.

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Plants can form various beneficial associations with soil microorganisms, such as associations with plant growth-promoting bacteria (PGPB). In this work, we report the full-genome sequence of the component of Mysorin biopreparation, identified as , consisting of a single 3.5-Mbp circular chromosome.

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Article Synopsis
  • Alternative splicing (AS) is a process that allows for the creation of different mRNA forms by varying pre-mRNA processing, playing a key role in gene expression regulation.
  • A study was conducted on garden pea roots in symbiosis with arbuscular mycorrhiza, using RNA sequencing to analyze the transcriptome and identify AS profiles, which were found to be largely similar between mycorrhizal and control roots.
  • Eight genes with AS events specific to the mycorrhizal roots were identified, including one related to splicing, suggesting that AS might contribute to the fine-tuning of gene expression during this symbiotic relationship.
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strain A1 is used in inoculation experiments with a wide range of pea ( L.) lines. In this study, we report the genome sequence of strain A1, consisting of a 5.

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Arbuscular mycorrhiza (AM) is known to be a mutually beneficial plant-fungal symbiosis; however, the effect of mycorrhization is heavily dependent on multiple biotic and abiotic factors. Therefore, for the proper employment of such plant-fungal symbiotic systems in agriculture, a detailed understanding of the molecular basis of the plant developmental response to mycorrhization is needed. The aim of this work was to uncover the physiological and metabolic alterations in pea ( L.

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Article Synopsis
  • Legume crops are a crucial source of food protein, and their productivity can be enhanced through symbiotic relationships with beneficial soil microorganisms like rhizobia and mycorrhizal fungi.
  • Recent research showed that a high-efficiency interaction pea line (K-8274) gained more productivity from these symbionts compared to a low-efficiency line (K-3358).
  • The study identified 111 proteins that differ in expression between the two lines, suggesting that the high-efficiency line benefits from prolonged seed maturation and enhanced cellular processes, highlighting the importance of this trait in breeding programs.
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Large collections of pea symbiotic mutants were accumulated in the 1990s, but the causal genes for a large portion of the mutations are still not identified due to the complexity of the task. We applied a Mapping-by-Sequencing approach including Bulk Segregant Analysis and Massive Analysis of cDNA Ends (MACE-Seq) sequencing technology for genetic mapping the gene of pea which controls the formation of symbioses with both nodule bacteria and arbuscular-mycorrhizal fungi. For mapping we developed an -population from the cross between pea line N24 carrying the mutant allele of and the wild type NGB1238 (=JI0073) line.

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Background: Samples pooling is a method widely used in studies to reduce costs and labour. DNA sample pooling combined with massive parallel sequencing is a powerful tool for discovering DNA variants (polymorphisms) in large analysing populations, which is the base of such research fields as Genome-Wide Association Studies, evolutionary and population studies, etc. Usage of overlapping pools where each sample is present in multiple pools can enhance the accuracy of polymorphism detection and allow identifying carriers of rare-variants.

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Sinorhizobium meliloti is a Gram-negative bacterium which fixes atmospheric nitrogen in symbiosis with Medicago spp. We report the draft genome sequence of S. meliloti strain CXM1-105, associated with nodules of Medicago sativa subsp.

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The inoculation of legume seeds with Sinorhizobium bacteria significantly improves pasture production. Here, we report the draft genome sequence of symbiotically efficient and salt-tolerant Sinorhizobium meliloti inoculant strain AK555, which substantially increases biomass yield of a number of Medicago sativa subsp. varia varieties, such as "Agniya," "Vega 87," and "Selena.

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Pea (Pisum sativum L.) is the oldest model object of plant genetics and one of the most agriculturally important legumes in the world. Since the pea genome has not been sequenced yet, identification of genes responsible for mutant phenotypes or desirable agricultural traits is usually performed via genetic mapping followed by candidate gene search.

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