Standard Versus Custom-Made Acetabular Implants in Revision Total Hip Arthroplasty.

Background: Today, various options are used for the reconstruction of acetabular bone loss in revision total hip arthroplasty (RTHA). The aim of the study was to compare the outcomes of using standard acetabular implants (SAIs) and custom-made acetabular implants (CMAIs) in RTHA in cases with extensive acetabular bone loss.

Methods: This was a comparative analysis of the results of 106 operations of RTHA performed during the period from January 2013 to December 2019.

The experimental study of tissue integration into porous titanium implants.

Introduction: Due to a lack of uniform shapes and sizes of bone defects in hip and knee joint pathology, their fixing could benefit from using individually manufactured 3D-printed highly porous titanium implants. The objective of this study was to evaluate the extent of bone and muscle tissue integration into porous titanium implants manufactured using additive technology.

Materials And Methods: Porous and non-porous titanium plates were implanted into the latissimus dorsi muscle and tibia of 9 rabbits.

Correction to: The optimization of the length of the lower limbs after hip arthroplasty.

Authors first and last names for the first and third authors have been interchanged. The correct presentation is given above.

The optimization of the length of the lower limbs after hip arthroplasty.

Background: The purpose of this research was to evaluate the effectiveness of hip joint arthroplasty in patients with different correction of the length of the legs and identifying factors that influence the outcome.

Material And Methods: We analyzed 93 patients operated upon with an initial shortening of the limb length by more than 3 cm. The difference in the length of the limbs ranged from 3 to 12 cm, which averaged 5 cm.

Influence of nucleotide modifications at the C2' position on the Hoogsteen base-paired parallel-stranded duplex of poly(A) RNA.

Polyadenylate (poly(A)) has the ability to form a parallel duplex with Hoogsteen adenine:adenine base pairs at low pH or in the presence of ammonium ions. In order to evaluate the potential of this structural motif for nucleic acid-based nanodevices, we characterized the effects on duplex stability of substitutions of the ribose sugar with 2'-deoxyribose, 2'-O-methyl-ribose, 2'-deoxy-2'-fluoro-ribose, arabinose and 2'-deoxy-2'-fluoro-arabinose. Deoxyribose substitutions destabilized the poly(A) duplex both at low pH and in the presence of ammonium ions: no duplex formation could be detected with poly(A) DNA oligomers.

Structural basis of interstrand cross-link repair by O-alkylguanine DNA alkyltransferase.

DNA interstrand cross-links (ICL) are among the most cytotoxic lesions found in biological systems. O-Alkylguanine DNA alkyltransferases (AGTs) are capable of removing alkylation damage from the O-atom of 2'-deoxyguanosine and the O-atom of thymidine. Human AGT (hAGT) has demonstrated the ability to repair an interstrand cross-linked duplex where two O-atoms of 2'-deoxyguanosine were tethered by a butylene (XLGG4) or heptylene (XLGG7) linkage.

Failure of the first step of two-stage revision due to polymicrobial prosthetic joint infection of the hip.

Background: The unsuccessful treatment of prosthetic joint infection (PJI) with two-stage revision leads to infection recurrence. The objectives of the study were to assess the clinical and demographic characteristics of patients with polymicrobial PJI, and to evaluate the role of the microbial profile involved in PJI in the risk of infection recurrence after the first step of two-stage revision surgery.

Materials And Methods: A retrospective analysis of 189 cases of culture-positive PJI following total hip replacement over a 5-year period was performed.

Risk factors and a prognostic model of hip periprosthetic infection recurrence after surgical treatment using articulating and non-articulating spacers.

Purpose: The purpose of this study was evaluation of the efficacy of the first step of a two-stage procedure for treatment of hip prosthetic joint infection (PJI) using articulating and non-articulating spacers as well as development of a prediction model and prognostic score for infection recurrence.

Methods: In a cohort of 217 patients treated for PJI of the hip, demographic characteristics, clinical symptoms, body temperature, body mass index (BMI), erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), white blood cell count (WBC), microbiological cultures and the type of the spacer used were retrospectively analyzed for association with the recurrence of PJI.

Results: Patients with infection recurrence had increased levels of ESR and CRP (P < 0.

Development of an ovine model of occlusive arterial injury for the evaluation of endovascular interventions.

Endovascular intervention for occlusive arterial trauma is becoming more common in clinical practice. The aim of this study is to present an ovine model of extremity arterial injury for use in future endovascular translational research. Animals under general anesthesia had their left superficial femoral artery exposed, which was bluntly injured over a 2-cm section using a hemostat and injection of air.

Experimental study of the installation acetabular component with uncoverage in arthroplasty patients with severe developmental hip dysplasia.

Purpose: The purpose of this study was stimation of optimal percentage of lateral uncoverage of the acetabular component during total hip arthroplasty for patients with severe developmental hip dysplasia.

Methods: Mathematical computer modeling based on the finite element technique and the mechanical experiment were performed. Critical values of uncoverage enabling safe primary fixation of acetabular component were estimated in designed models.

NMR structure of an ethylene interstrand cross-linked DNA which mimics the lesion formed by 1,3-bis(2-chloroethyl)-1-nitrosourea.

The bisalkylating agent 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU), used in cancer chemotherapy to hinder cellular proliferation, forms lethal interstrand cross-links (ICLs) in DNA. BCNU generates an ethylene linkage connecting the two DNA strands at the N1 atom of 2'-deoxyguanosine and N3 atom of 2'-deoxycytidine, which is a synthetically challenging probe to prepare. To this end, an ICL duplex linking the N1 atom of 2'-deoxyinosine to the N3 atom of thymidine via an ethylene linker was devised as a mimic.

An interaction map of endoplasmic reticulum chaperones and foldases.

Chaperones and foldases in the endoplasmic reticulum (ER) ensure correct protein folding. Extensive protein-protein interaction maps have defined the organization and function of many cellular complexes, but ER complexes are under-represented. Consequently, chaperone and foldase networks in the ER are largely uncharacterized.

Structural basis of defects in the sacsin HEPN domain responsible for autosomal recessive spastic ataxia of Charlevoix-Saguenay (ARSACS).

Sacsin is a 520-kDa protein mutated in the early-onset neurodevelopmental and neurodegenerative disease autosomal recessive spastic ataxia of Charlevoix-Saguenay (ARSACS). The C terminus of the protein contains an HEPN (higher eukaryotes and prokaryotes nucleotide-binding) domain of unknown function. Here, we determined the high-resolution 1.

Structure of the catalytic a(0)a fragment of the protein disulfide isomerase ERp72.

Protein disulfide isomerases (PDIs) are responsible for catalyzing the proper oxidation and isomerization of disulfide bonds of newly synthesized proteins in the endoplasmic reticulum (ER). The ER contains many different PDI-like proteins. Some, such as PDI, are general enzymes that directly recognize misfolded proteins while others, such as ERp57 and ERp72, have more specialized roles.

Soluble fullerene derivative in liquid crystal: polymer composites and their impact on photorefractive grating efficiency and resolution.

By using soluble fullerene derivative [60]PCBM, we improved photorefractive efficiency in polymer-liquid crystal composites in comparison to previous works on similar materials. We show the effect of polymer network results in resolution and bandwidth improvements compared to pure liquid crystals. This is explained by the introduction of a charge trapping mechanism, providing a memory effect for the composite.

Solution structure of the bb' domains of human protein disulfide isomerase.

Protein disulfide isomerase is the most abundant and best studied of the disulfide isomerases that catalyze disulfide bond formation in the endoplasmic reticulum, yet the specifics of how it binds substrate have been elusive. Protein disulfide isomerase is composed of four thioredoxin-like domains (abb'a'). Cross-linking studies with radiolabeled peptides and unfolded proteins have shown that it binds incompletely folded proteins primarily via its third domain, b'.

The discovery of small molecule chemical probes of Bcl-X(L) and Mcl-1.

A tetrahydroaminoquinoline-based library was generated with the goals of finding small molecule modulators of protein-protein interactions. Several library members as well as other related intermediates were tested for their ability to bind to Bcl-X(L) and Mcl-1 by in silico and (15)N NMR studies. The NMR study led to the identification of the tetrahydroaminoquinoline-based nude scaffold, 7 as a weak binder (K(d)=200 microM for Bcl-X(L) and K(d)=300 microM for Mcl-1) to both proteins.

Resonant gratings in planar Grandjean cholesteric composite liquid crystals.

We propose to induce a two-dimensional (2D) periodic modulation structure into a planar Grandjean cholesteric liquid crystal (CLC) to demonstrate the intrinsic 2D photonic crystal properties of such materials. The structure combines a thin transmission grating and a Bragg reflective grating. One advantage of using CLC is the intrinsic high quality Bragg structure, which can be modulated by an electric field: shifting the wavelength band edge by changing the applied field.

1H, 13C and 15N resonance assignments of the bb' domains of human protein disulfide isomerase.

Protein disulfide isomerase (PDI) participates in protein folding and catalyses formation of disulfide bonds. The b' domain of human PDI contributes to binding unfolded proteins; its structure is stabilized by the b domain. Here, we report NMR chemical shift assignments for the bb' fragment.

Solution structure of the catalytic domain of RICH protein from goldfish.

Regeneration-induced CNPase homolog (RICH) is an axonal growth-associated protein, which is induced in teleost fish upon optical nerve injury. RICH consists of a highly acidic N-terminal domain, a catalytic domain with 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) activity and a C-terminal isoprenylation site. In vitro RICH and mammalian brain CNPase specifically catalyze the hydrolysis of 2',3'-cyclic nucleotides to produce 2'-nucleotides, but the physiologically relevant in vivo substrate remains unknown.

Heat-induced dimerization of BCL-xL through alpha-helix swapping.

The dimerization of anti-apoptotic BCL-xL by three-dimensional domain swapping has recently been discovered at alkaline pH; however, the high energetic barrier between the dimer and monomer forms of BCL-xL prevents them from interconverting at room temperature and neutral pH. Here, we demonstrate that BCL-xL dimers can be easily prepared by heating concentrated protein above 50 degrees C. The 38 kDa BCL-xL dimer was fully characterized by multi-resonance nuclear magnetic resonance (NMR) spectroscopy, and the mechanism of dimerization by alpha-helix swapping was confirmed.