Ixodes ricinus ticks (Acari: Ixodidae): vectors for Lyme disease spirochetes in Romania.

In this study 1,868 questing Ixodes ricinus ticks (nymphs and adults), collected in six sites from three counties--Giurgiu, Sibiu, and Tulcea--in Romania, were examined by polymerase chain reaction (PCR) followed by reverse line blot (RLB) for detection of Borrelia burgdorferi sensu lato presence. The bacteria were found in 18% of the investigated ticks. The prevalence of infection did not differ significantly between nymphs (19.

First report of lyme disease spirochetes in ticks from Romania (Sibiu County).

We examined 200 questing Ixodes ricinus ticks (nymphs and adults) collected in three different sites in Sibiu County, Romania by polymerase chain reaction (PCR) followed by reverse line blot (RLB) for Borrelia burgdorferi s.l..