Publications by authors named "Alexandre Boubegtitene"
Article Synopsis
- Recent studies have discovered a new 5' to 3' pathway for mRNA decay called Co-Translational mRNA Decay (CTRD), challenging the previous understanding that mRNA decay occurs only in the cytosol after leaving ribosomes.
- Researchers created a specific Arabidopsis line (XRN4ΔCTRD) to analyze the role of CTRD in mRNA turnover, finding that mRNA is generally more stable in the root compared to the shoot.
- The absence of CTRD in the shoot led to significant mRNA stabilization, while in the root, decay rates increased, indicating that XRN4-dependent cytosolic decay compensates for the lack of CTRD and emphasizing its importance in
mRNA decay is an important process in post-transcriptional regulation; in addition, it plays a crucial role in plant development and response to stress. The development of new tools to quantify mRNA decay intermediates is thus important to better characterize the dynamic of mRNA decay in various conditions. Here, we applied droplet digital PCR (ddPCR), a recent and precise PCR technology, to determine mRNA half-life in Arabidopsis seedlings.
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