Involvement of UV-C-induced genomic instability in stimulation рlant long-term protective reactions.

The study of the mechanisms affecting single stress factor impact on long-term metabolic rearrangements is necessary for understanding the principles of plant protective reactions. The objective of the study was to assess the involvement of UV-C-induced genomic instability in induction рlant long-term protective reactions. The study was carried out on two genotypes of chamomile, Perlyna Lisostepu (PL) variety and its mutant, using UV-C pre-sowing seed radiation exposure at dose levels 5-15 kJ/m.

Relationship of radiation-induced genomic instability and antioxidant production in the chamomile plant.

Purpose: To verify the hypothesis about the preservation of signs of radiation-induced genomic instability at the flowering stage of the chamomile plant after pre-sowing seed irradiation, the interaction of dose-dependent changes in the level of DNA damage and stimulation of antioxidant production.

Materials And Methods: The study was carried out on two genotypes of chamomile, Perlyna Lisostepu variety and its mutant, using pre-sowing seed radiation exposure at dose levels 5-15 Gy. Studies of the rearrangement of the primary DNA structure of under different doses were studied on plant tissues at the flowering stage using - ISSR and RAPD DNA markers.

Epigenetic factors of individual radiosensitivity and adaptive capacity.

Studying the relationship between epigenetic variability with different individual radiosensitivity and adaptive capacity. Using a simple and convenient experimental model - maize seedlings with different germination terms and epigenetic patterns - the hypothesis was tested that homogeneous genetically but epigenetic different organisms have different radiosensitivity and radioadaptive capacity. Differences in the DNA methylation profiles of individual subpopulations of seedlings were used as a marker of epigenetic differences and the yield of chromosomal aberration was used as an indicator of DNA vulnerability and its changes under different UV-C irradiation modes.