Controlling Pericellular Oxygen Tension in Cell Culture Reveals Distinct Breast Cancer Responses to Low Oxygen Tensions.

In oxygen (O)-controlled cell culture, an indispensable tool in biological research, it is presumed that the incubator setpoint equals the O tension experienced by cells (i.e., pericellular O).

Controlling pericellular oxygen tension in cell culture reveals distinct breast cancer responses to low oxygen tensions.

Oxygen (O) tension plays a key role in tissue function and pathophysiology. O-controlled cell culture, in which the O concentration in an incubator's gas phase is controlled, is an indispensable tool to study the role of O . For this technique, it is presumed that the incubator setpoint is equal to the O tension that cells experience (.

De Novo Self-Assembling Peptides Mediate the Conversion of Temozolomide and Delivery of a Model Drug into Glioblastoma Multiforme Cells.

Glioblastoma multiforme (GBM) is the most aggressive central nervous system tumor, and standard treatment, including surgical resection, radiation, and chemotherapy, has not significantly improved patient outcomes over the last 20 years. Temozolomide (TMZ), the prodrug most commonly used to treat GBM, must pass the blood-brain barrier and requires a basic pH to convert to its active form. Due to these barriers, less than 30% of orally delivered TMZ reaches the central nervous system and becomes bioactive.

Factors Affecting Secondary and Supramolecular Structures of Self-Assembling Peptide Nanocarriers.

Self-assembling peptides are a popular vector for therapeutic cargo delivery due to their versatility, tunability, and biocompatibility. Accurately predicting secondary and supramolecular structures of self-assembling peptides is essential for de novo peptide design. However, computational modeling of such assemblies is not yet able to accurately predict structure formation for many peptide sequences.