Engineering the migration and attachment behaviour of primary dermal fibroblasts.

The availability of primary cells present in pathological conditions is often very limited due to stringent ethical regulation and patient consent. One such condition is chronic wounds, where dermal fibroblasts show a deficient migration. In vitro models with cellular tools that mimic the in vivo scenario would be advantageous to test new therapies for these challenging wounds.

The importance of factorial design in tissue engineering and biomaterials science: Optimisation of cell seeding efficiency on dermal scaffolds as a case study.

This article presents a case study to show the usefulness and importance of using factorial design in tissue engineering and biomaterials science. We used a full factorial experimental design (2 × 2 × 2 × 3) to solve a routine query in every biomaterial research project: the optimisation of cell seeding efficiency for pre-clinical in vitro cell studies, the importance of which is often overlooked. In addition, tissue-engineered scaffolds can be cellularised with relevant cell type(s) to form implantable tissue constructs, where the cell seeding method must be reliable and robust.

[Progesterone receptor's profile and the effect of the hormone and its derivatives on invasiveness and MMP2 secretion in endometrial carcinoma cell lines].

Introduction: Progesterone inhibits endometrial proliferation and is used for the treatment of early stage endometrial carcinoma in women interested in fertility preservation or for advanced or recurrent disease. Better responses and prognosis were documented in women who are progesterone receptor positive. The receptor has 2 main isoforms and the ratio between these two is responsible for progesterone activity

Objectives: To evaluate the effect of progesterone and its derivative on invasion and matrix metalloproteinase 2 (MMP2) secretion in two endometrial carcinoma cell lines having different progesterone receptor isoform profiles.