Publications by authors named "Alexandra H Springwald"
Article Synopsis
- The study explores how loading siRNA into gelatin microparticles (cGM) can enhance targeted therapies for bone health.
- The researchers combined these siRNA-loaded cGM with human mesenchymal stem cells (hMSC) to create microtissues, leading to increased alkaline phosphatase activity after silencing chordin, which inhibits bone formation.
- The microtissues showed improved bone-related activity in a co-culture model with blood cells, suggesting they could potentially promote bone growth and maintain bone health.
The aim of this study was the evaluation of cross-linked gelatin microparticles (cGM) as substrates for osteogenic cell culture to assemble 3D microtissues and their use as delivery system for siRNA to cells in these assemblies. In a 2D transwell cultivation system, we found that cGM are capable to accumulate calcium ions from the surrounding medium. Such a separation of cGM and SaOS-2 cells consequently led to a suppressed matrix mineral formation in the SaOS-2 culture on the well bottom of the transwell system.
View Article and Find Full Text PDFToward the next generation of nerve guidance conduits (NGCs), novel biomaterials and functionalization concepts are required to address clinical demands in peripheral nerve regeneration (PNR). As a biological polymer with bioactive motifs, gelatinous peptides are promising building blocks. In combination with an anhydride-containing oligomer, a dual-component hydrogel system (cGEL) was established.
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