Publications by authors named "Alexandra Emmanuel"
Article Synopsis
- Immunoglobulin G (IgG) proteins have diverse variable domains that help protect against foreign antigens, with specific polymorphisms in their constant regions impacting precise sequence identification.
- The study aimed to validate proteomic results by sequencing DNA fragments from ten individuals, selected based on Gm allotype polymorphism, covering IgG gene diversity.
- Results showed a 95% match between IgG alleles identified through mass spectrometry and those determined through genomic sequencing, supporting the use of proteomic methods for diagnostic purposes, particularly in congenital infection cases.
To better understand the role of immunocompetent hosts in the diffusion of Pneumocystis in the environment, airborne shedding of Pneumocystis carinii in the surrounding air of experimentally infected Sprague Dawley rats was quantified by means of a real-time PCR assay, in parallel with the kinetics of P. carinii loads in lungs and specific serum antibody titres. Pneumocystis-free Sprague Dawley rats were intratracheally inoculated at day 0 (d0) and then followed for 60 days.
View Article and Find Full Text PDF