Sprayable Biocide-Free Polyurethane Paint that Reduces Biofouling and Facilitates Removal of Pathogenic Bacteria from Surfaces.

The ability to prevent bacterial adhesion on surfaces and to facilitate the removal of bacteria once they have already contaminated or colonized a surface is important in a broad range of fundamental and applied contexts. The work reported here sought to characterize the physicochemical properties of a family of biocide-free hydrophobic polyurethane coatings containing polysiloxane segments and evaluate their ability to mitigate bacterial fouling and/or facilitate subsequent surface cleaning after exposure to pathogenic bacteria. We developed benchtop microbiological assays to characterize surface fouling and subsequent removal of bacteria after repeated (i) short-term intermittent physical contact with and (ii) longer-term continuous flow-based contact with liquid growth media containing either or , two common Gram-positive or Gram-negative bacterial pathogens, respectively.