You get what you screen for: on the value of fermentation characterization in high-throughput strain improvements in industrial settings.

While design and high-throughput build approaches in biotechnology have increasingly gained attention over the past decade, approaches to test strain performance in high-throughput have received less discussion in the literature. Here, we describe how fermentation characterization can be used to improve the overall efficiency of high-throughput DBTAL (design-build-test-analyze-learn) cycles in an industrial context. Fermentation characterization comprises an in-depth study of strain performance in a bioreactor setting and involves semi-frequent sampling and analytical measurement of substrates, cell densities and viabilities, and (by)products.