Publications by authors named "Alexander V Troitskiy"
We developed recombinant variants of oncolytic vaccinia virus LIVP strain expressing interleukin-15 (IL-15) or its receptor subunit alpha (IL-15Rα) to stimulate IL-15-dependent immune cells. We evaluated their oncolytic activity either alone or in combination with each other and using the murine CT26 colon carcinoma and 4T1 breast carcinoma models. We demonstrated that the admixture of these recombinant variants could promote the generation of the IL-15/IL-15Rα complex.
View Article and Find Full Text PDFThis review summarizes the current understanding of the interaction between circadian rhythms of gene expression and epigenetic clocks characterized by the specific profile of DNA methylation in CpG-islands which mirror the senescence of all somatic cells and stem cells in particular. Basic mechanisms of regulation for circadian genes CLOCK-BMAL1 as well as downstream clock-controlled genes (ССG) are also discussed here. It has been shown that circadian rhythms operate by the finely tuned regulation of transcription and rely on various epigenetic mechanisms including the activation of enhancers/suppressors, acetylation/deacetylation of histones and other proteins as well as DNA methylation.
View Article and Find Full Text PDFArticle Synopsis
- * Researchers created spidroin-based electrospun mats modified with ECM peptide motifs (RGD, IKVAV, VAEIDGIEL) to analyze their effects on the behavior of directly reprogrammed neural precursor cells (drNPCs).
- * Findings show that these modified mats help maintain the stemness of drNPCs while also influencing differentiation, with RGD promoting fewer but longer neurite-forming neurons, and IKVAV leading to more neurons but with shorter neurites, while still preserving neuroglial progenitor
Article Synopsis
- The study focuses on addressing key challenges in developing regenerative therapies for spinal cord injuries (SCI) by using directly reprogrammed neural precursor cells (drNPCs) as a potential solution for safe and effective treatment.
- Researchers performed intraspinal transplantation of drNPCs in seven non-human primates with complete thoracic SCI, comparing the results against a control group receiving a vehicle injection.
- Findings showed significant recovery in hindlimb function, neurological assessments, and maintained cell multipotency, indicating that drNPC transplantation is a safe and promising approach for enhancing spinal cord function post-injury.