Three titanium alloys with 0.5, 6, and 9 wt.% iron were investigated, and the samples were pre-annealed in three different regions of the Ti-Fe phase diagram, namely β, α+β, and α+FeTi.
View Article and Find Full Text PDFThe complexities of translational strategies make this stage of implementing genetic information one of the most challenging to comprehend and, simultaneously, perhaps the most engaging. It is evident that this diverse range of strategies results not only from a long evolutionary history, but is also of paramount importance for refining gene expression and metabolic modulation. This notion is particularly accurate for organisms that predominantly exhibit biochemical and physiological reactions with a lack of behavioural ones.
View Article and Find Full Text PDFThe β-type Ti-42Nb alloy has been successfully manufactured from pre-alloyed powder using the E-PBF method for the first time. This study presents thorough microstructural investigations employing diverse methodologies such as EDS, XRD, TEM, and EBSD, while mechanical properties are assessed using UPT, nanoindentation, and compression tests. Microstructural analysis reveals that Ti-42Nb alloy primarily consisted of the β phase with the presence of a small amount of nano-sized α″-martensite formed upon fast cooling.
View Article and Find Full Text PDFA Zr-doped CaO sorbent for high-temperature CO capture was fabricated using electrospinning. The nanofiber sorbent with an average filament diameter of about 160 nm is characterized by an initial CO uptake capacity of 12.1 mmol/g, a specific surface area of 79 m/g, an indentation Young's modulus of 520 MPa, and a hardness of 1.
View Article and Find Full Text PDFIn this work the high-entropy alloy studied contained six components, Ti/Zr/Hf/Mo/Cr/Co, and three phases, namely one phase with body-centered cubic lattice (BCC) and two Laves phases C14 and C15. A series of annealings in the temperature range from 600 to 1000 °C demonstrated not only a change in the microstructure of the TiZrHfMoCrCo alloy, but also the modification of phase composition. After annealing at 1000 °C the BCC phase almost fully disappeared.
View Article and Find Full Text PDFBackground: RNA-seq has become a standard technology to quantify mRNA. The measured values usually vary by several orders of magnitude, and while the detection of differences at high values is statistically well grounded, the significance of the differences for rare mRNAs can be weakened by the presence of biological and technical noise.
Results: We have developed a method for cleaning RNA-seq data, which improves the detection of differentially expressed genes and specifically genes with low to moderate transcription.
Background: For the needs of modern biotechnology, a quantitative approach to the control of regulatory elements at all stages of gene expression has long become indispensable. Such a control regime is impossible without a quantitative analysis of the role of each regulatory element or pattern used. Therefore, it seems important to modify and develop the accuracy, reproducibility, and availability of methods for quantifying the contribution of each regulatory code to the implementation of genetic information.
View Article and Find Full Text PDFThe nanofibrous CaO sorbent for high-temperature CO capture was fabricated by the calcination of electrospun composite filaments containing calcium acetylacetonate and polyacrylonitrile as a calcium-oxide precursor and a binder polymer, respectively. The calcination was carried out in air to prevent PAN carbonization and to obtain pure CaO nanofibers. The resulting mats of CaO nanofibers with the average diameter of 130 nm were characterized by a specific surface area of 31 m/g, a CO-uptake capacity of 16.
View Article and Find Full Text PDFThis paper describes an experimental study of the relationships between thermal diffusivity and mechanical characteristics including Brinell hardness, microhardness, and Young's modulus of common pine ( L.), pedunculate oak ( L.), and small-leaf lime ( Mill.
View Article and Find Full Text PDFZirconia nanofiber mats containing filaments with the average diameter of less than 100 nm were fabricated. It is found that the hardness and Young's modulus of the mats are sensitive to the microstructure, phase composition and average diameter of the zirconia nanofibers. The hardness and Young's modulus of the prepared zirconia nanofiber mats vary from 0.
View Article and Find Full Text PDFBackground: Since the beginning of the use of reporter proteins for expression analysis, a variety of approaches have been developed and proposed; both qualitative and quantitative. The lack of simple methods for direct observation of gene expression in living organisms makes it necessary to continue to propose new methods. In this work, we consider a method for the quantitative analysis of the expression of thermostable lichenase from Clostridium thermocellum used as a sensitive reporter protein.
View Article and Find Full Text PDFWet high-energy milling and uniaxial pressing are used to fabricate CaO-stabilized tetragonal zirconia polycrystalline ceramic (Ca-TZP) with decent mechanical characteristics, i.e., a hardness of 11.
View Article and Find Full Text PDFACS Biomater Sci Eng
March 2020
In this study, hybrid composites based on β-alloy Ti-Nb and oxide nanotubes (NTs) have been successfully prepared. NTs of different sizes were grown on Ti-Nb substrates with different Nb contents (5, 25, and 50 wt %) via electrochemical anodization at 30 and 60 V. Scanning electron microscopy imaging revealed that vertically aligned nanotubular structures form on the surface of Ti-Nb alloy substrates and influence Nb content in alloys based on NT length.
View Article and Find Full Text PDFThe mats of yttria-stabilized tetragonal zirconia nanofibers were prepared using electrospinning. The effect of calcination temperature in the range of 600-1200 °C on their microstructure, phase composition and mechanical properties was investigated. Phase composition of the nanofibers did not change in all ranges of the calcination temperatures, while the average grain size increased from 8 to 39 nm.
View Article and Find Full Text PDFA large data array on plant gene expression accumulated thanks to comparative omic studies directs the efforts of researchers to the specific or fine effects of the target gene functions and, as a consequence, elaboration of relatively simple and concurrently effective approaches allowing for the insight into the physiological role of gene products. Numerous studies have convincingly demonstrated the efficacy of transient expression strategy for characterization of the plant gene functions. The review goals are (i) to consider the advantages and limitations of different plant systems and methods of transient expression used to find out the role of gene products; (ii) to summarize the current data on the use of the transient expression approaches for the insight into fine mechanisms underlying the gene function; and (iii) to outline the accomplishments in efficient transient expression of plant genes.
View Article and Find Full Text PDFInt J Mol Sci
December 2018
The main specific features of β-1,3-1,4-glucanases (or lichenases, EC 3.2.1.
View Article and Find Full Text PDFWe have proposed and tested a method for characterization of the signal sequences and determinations of target protein localization in a plant cell. This method, called the AgI-PrI, implies extraction of protoplasts from plant tissues after agroinfiltration. The suggested approach combines the advantages of two widely used methods for transient gene expression in plants-agroinfiltration and transfection of isolated protoplasts.
View Article and Find Full Text PDFBeryllium, being one of the most transparent materials to X-ray radiation, has become the material of choice for X-ray optics instrumentation at synchrotron radiation sources and free-electron laser facilities. However, there are concerns due to its high toxicity and, consequently, there is a need for special safety regulations. The authors propose to apply protective coatings in order to seal off beryllium from the ambient atmosphere, thus preventing degradation processes providing additional protection for users and prolonging the service time of the optical elements.
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