Spectroscopy study of dimerization of fluorone dyes in AOT reverse micelles.

The dimerization processes and its thermodynamic parameters of fluoronic dyes (fluorescein (F), eosin (E), erythrosine (ER), bengal rose (BR)) in reverse micelles of AOT with different hydrodynamic radius R are studied. The dimerization constants and its efficiency (the degree of dimerization of dye molecules (1-X)) were determined from the experimental data. It is found that an increase in the intercombination conversion due to the heavy atom effect leads to an increase of the value of (1-X).

Tyrosine fluorescence probing of the surfactant-induced conformational changes of albumin.

Tyrosine fluorescence in native proteins is known to be effectively quenched, whereas its emission increases upon proteins' unfolding. This suggests that tyrosine fluorescence could be exploited for probing structural rearrangements of proteins in addition to the extensively used tryptophan emission. We studied the possibility of using tyrosine fluorescence as an indicator of surfactant-induced conformational changes in albumins.

Raman spectroscopy and fluorescence analysis in investigation of the protective action of ischemic preconditioning at ischemic insult by estimation of damage of low-density lipoprotein of blood.

Objective And Background Data: In recent years medical researchers have sought a method of ischemic preconditioning, which is training an organ for prolonged total ischemia by inducing short periods of ischemia. In this work we detail research on this modality, conducted before ischemic insult in Wistar rats, in order to quantify the amount of damage done to low-density lipoprotein (LDL) in the blood. In previous research we used optical spectral methods to ascertain changes in LDL in the blood of animals after ischemic insult.