Publications by authors named "Alexander M Shuppara"
Article Synopsis
- Real-world organisms face multiple stressors simultaneously, but lab studies usually focus on single stressors in simplified conditions.
- This study uses microfluidics to apply both physical (shear flow) and chemical (hydrogen peroxide) stressors to the bacteria Pseudomonas aeruginosa, revealing that flow significantly enhances the effectiveness of HO on bacterial growth.
- The findings show that natural levels of these stressors interact in ways that limit bacterial movement and survival, emphasizing the importance of studying multiple stressors to better understand their true effects.
Article Synopsis
- Laboratory studies often focus on single stressors, while in nature, organisms face multiple stressors at once.
- This research used a microfluidic approach to combine physical (shear flow) and chemical (H2O2) stressors on a human pathogen, revealing that flow greatly enhances the effectiveness of H2O2 against bacteria.
- Findings indicate that natural levels of H2O2 and flow work together to impede bacterial growth, suggesting that studying multiple stressors is crucial for understanding real-life biological interactions.
Article Synopsis
- * Researchers utilized biophysical simulations and microfluidic experiments, finding that increased fluid flow helps overcome bacterial resistance to various antimicrobials by enhancing their delivery.
- * The study highlights the importance of combining physical flow with chemical dosage in developing effective antimicrobials, suggesting that this strategy could be crucial in combating rising antimicrobial resistance.
Article Synopsis
- * Recent interdisciplinary research has shed light on how flow affects various bacterial responses, including adhesion, movement, colonization, and gene expression.
- * The review focuses on four different bacterial species and outlines experimental methods to study their behavior in flow, highlighting that not all responses are due to shear forces and emphasizing flow-sensitive signaling in bacteria.
Proc Natl Acad Sci U S A
March 2023
Article Synopsis
- - Cells in natural environments are affected by fluid flow, but most lab experiments, which use batch cell culture, overlook this factor in studying cell behavior and physiology.
- - Research using microfluidics and single-cell imaging reveals that the combination of fluid flow (shear rate) and chemical stress from hydrogen peroxide affects how human pathogens respond at a genetic level, in ways not seen in standard lab settings.
- - High shear rates in flowing conditions can significantly increase cells' sensitivity to low levels of hydrogen peroxide, aligning experimental conditions more closely with those in the human bloodstream, which helps explain how bacteria adapt to their natural environments.
