Influence of maternal rats' hypothyroidism on morpho-functional peculiarities and glycome of progeny skin.

We studied the influence of maternal hypothyroidism on progeny skin morphogenesis by means of histological, histochemical and lectin-histochemical methods. Hypothyroid conditions in rats were achieved by daily food supplementation with antithyroid drug Mercazolil. The experiment was conducted on 10 control and 10 hypothyroid rats, which delivered 70 and 46 offsprings, respectively.

Oligomannose-Rich Membranes of Dying Intestinal Epithelial Cells Promote Host Colonization by Adherent-Invasive .

A novel mechanism is revealed by which clinical isolates of adherent-invasive (AIEC) penetrate into the epithelial cell layer, replicate, and establish biofilms in Crohn's disease. AIEC uses the FimH fimbrial adhesin to bind to oligomannose glycans on the surface of host cells. Oligomannose glycans exposed on early apoptotic cells are the preferred binding targets of AIEC, so apoptotic cells serve as potential entry points for bacteria into the epithelial cell layer.

Lectin purification from carp roe (Cyprinus carpio L.), investigation of its carbohydrate specificity and application in histochemistry.

A method of lectin purification from carp roe (CCRA) was elaborated, which includes affinity chromatography on cross-linked ovomucoid and copolymers of polyvinyl alcohol and blood group-specific substances. That allowed obtaining lectin with electrophoretic purity and yielding of ˜ 42 mg÷kg roe. Electrophoresis in 15% polyacrylamide gel in the presence of β-mercaptoethanol showed one band with molecular mass ˜ 15 kDa, whereas in the absence of β-mercaptoethanol, CCRA exposed band with molecular mass ˜ 60 kDa.

Lectin purification from fruiting bodies of brown roll-rim fungus, Paxillus involutus (Fr.) Fr., and its application in histochemistry.

A lectin (agglutinin) from fresh fruit bodies of the brown roll-rim fungus [Paxillus involutus (Fr.) Fr.] has been purified with output approx.

Visualization of melanoma tumor with lectin-conjugated rare-earth doped fluoride nanocrystals.

Aim: To develop specific fluorescent markers for melanoma tumor visualization, which would provide high selectivity and reversible binding pattern, by the use of carbohydrate-recognizing proteins, lectins, combined with the physical ability for imaging deep in the living tissues by utilizing red and near infrared fluorescent properties of specific rare-earth doped nanocrystals (NC).

Methods: B10F16 melanoma cells were inoculated to C57BL/6 mice for inducing experimental melanoma tumor. Tumors were removed and analyzed by lectin-histochemistry using LABA, PFA, PNA, HPA, SNA, GNA, and NPL lectins and stained with hematoxylin and eosin.

Diabetic alteration versus postnatal maturation of rat kidney glycoconjugates--comparative detection by lectin probes.

A panel of ten lectins with different carbohydrate specificities, including three original lectin preparations (MPFA, LABA, and LVA), was used for the investigation of rat kidney glycoconjugate remodeling during postnatal morphogenesis, and the findings were compared with the impairments seen in streptozotocin-induced diabetic nephropathy. Postnatal morphogenesis was accompanied by the accumulation and generalization of DMan, LFuc, and NeuNAc, with simultaneous reduction of DGal and DGalNAc sugar determinants and enhanced heterogeneity of renal microstructure. The most significant redistribution of lectin receptor sites was detected between postnatal days 1 and 20.

Lectin receptor sites during postnatal osteogenesis in guinea pigs.

Background: Osteoporosis and its complications have become widespread, affecting large portions of the world's population. More advanced information is needed on these pathologies to expand the possibilities for pathogenetic therapy.

Objectives: Lectin histochemistry methods offer new insights into the structure of tissue carbohydrates and their rearrangement under physiological and pathological conditions.

Structural, functional, and lectin histochemical characteristics of rat ovaries and endometrium in experimental hyper- and hypothyroidism.

Twenty lectins with different carbohydrate affinities, including five new lectins purified from fungi,were used for histochemical examination of carbohydrate determinants in rat ovaries and endometrium under experimental hyper- and hypothyroidism. In the ovaries of control rats, lectin binding was detected predominantly in the zona pellucida and corona radiata of growing follicles, luteocytes, and leucocytes. Within the endometrium,lectins reacted strongly with the luminal surface, epitheliocytes and secretions of the uterine glands,pre-decidual cells, leucocytes, and collagen fibers.

Persistent accumulation of 4-hydroxynonenal-protein adducts in gastric mucosa after Helicobacter pylori eradication.

Recent studies indicate that oxidative stress caused by Helicobacter pylori and insufficient host antioxidant defense could play important role in pathogenesis of gastrointestinal ulcerations. By specific monoclonal antibodies we have detected weak presence of the major lipid peroxidation bioactive marker 4-hydroxynonenal (HNE) in healthy human gastric mucosa, which strongly increased in case of H. pylori-associated peptic ulcer.

Protein kinase C phosphorylates ribosomal protein S6 kinase betaII and regulates its subcellular localization.

The ribosomal protein S6 kinase (S6K) belongs to the AGC family of Ser/Thr kinases and is known to be involved in the regulation of protein synthesis and the G(1)/S transition of the cell cycle. There are two forms of S6K, termed S6Kalpha and S6Kbeta, which have cytoplasmic and nuclear splice variants. Nucleocytoplasmic shuttling has been recently proposed for S6Kalpha, based on the use of the nuclear export inhibitor, leptomycin B.