Publications by Alexander J Millington

Publications by authors named "Alexander J Millington"

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N-linked glycosylation of VWF modulates its interaction with ADAMTS13.

Thomas A J McKinnon Alain C K Chion Alexander J Millington David A Lane Mike A Laffan

Blood

March 2008

We examined the role of N-linked glycan structures of VWF on its interaction with ADAMTS13. PNGase F digestion followed by lectin analysis demonstrated that more than 90% of VWF N-linked glycan chains could be removed from the molecule (PNG-VWF) without disruption of its multimeric structure or its ability to bind to collagen. PNG-VWF had an approximately 4-fold increased affinity for ADAMTS13 compared with control VWF.

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- Alexander J Millington's research focuses on the biochemical interactions of von Willebrand Factor (VWF) and its modulation through post-translational modifications, specifically N-linked glycosylation.
- His study published in "Blood" analyzed how the removal of N-linked glycans from VWF affects its interaction with the protease ADAMTS13, revealing significant insights into their binding dynamics.
- The findings indicated that VWF with removed glycan structures exhibited a fourfold increase in binding affinity to ADAMTS13 while maintaining its functional integrity, highlighting the critical role of glycosylation in VWF's biological interactions.

Publications by authors named "Alexander J Millington"

N-linked glycosylation of VWF modulates its interaction with ADAMTS13.

Synopsis of recent research by authors named "Alexander J Millington"