High-accuracy measurements of OH reaction rate constants and IR absorption spectra: CH2=CF-CF3 and trans-CHF=CH-CF3.

Rate constants for the gas phase reactions of OH radicals with two isomers of tetrafluoropropene, CH(2)=CF-CF(3) (k(1)) and trans-CHF=CH-CF(3) (k(2)); were measured using a flash photolysis resonance-fluorescence technique over the temperature range 220 to 370 K. The Arrhenius plots were found to exhibit a noticeable curvature. The temperature dependences of the rate constants are very weak and can be represented by the following expressions over the indicated temperature intervals: k(1)(220-298 K) = 1.

Particulate platform for bioluminescent immunosensing.

The present study examines pyruvate kinase-conjugated antibodies for potential use in ELISA applications. The conjugates had an acceptable stability, and the coupling inflicted only minor impairment on the kinase activity. To mimic the setup of an immunoassay under development, a test antigen (BSA) was attached to polystyrene nanoparticles.

A study of systems for delivering antigens and plasmid DNA for intranasal immunization against tick-borne encephalitis virus.

Our previous studies indicated the possibility for some neurotropic viruses to spread into the brain of immune animals through the olfactory pathway. Thus, nasal mucosa in the olfactory region is likely to be a promising target for mucosal immunization to protect the CNS from neurotropic viral infections. THE MAIN IDEA OF THE RESEARCH: Intranasal immunization inducing mucosal and systemic immune responses blocks the propagation of neurotropic virus into the brain via the olfactory pathway and neutralizes the multiplication of virus in visceral organs, allowing more effective protection against neurotropic infections transmitted by bloodsucking arthropods to be achieved.