Publications by authors named "Alexander G Georgiev"
The pan-eukaryotic endoplasmic reticulum (ER) membrane protein Arv1 has been suggested to play a role in intracellular sterol transport. We tested this proposal by comparing sterol traffic in wild-type and Arv1-deficient Saccharomyces cerevisiae. We used fluorescence microscopy to track the retrograde movement of exogenously supplied dehydroergosterol (DHE) from the plasma membrane (PM) to the ER and lipid droplets and high performance liquid chromatography to quantify, in parallel, the transport-coupled formation of DHE esters.
View Article and Find Full Text PDFSterol transport between the endoplasmic reticulum (ER) and plasma membrane (PM) occurs by an ATP-dependent, non-vesicular mechanism that is presumed to require sterol transport proteins (STPs). In Saccharomyces cerevisiae, homologs of the mammalian oxysterol-binding protein (Osh1-7) have been proposed to function as STPs. To evaluate this proposal we took two approaches.
View Article and Find Full Text PDFArticle Synopsis
- The study focuses on creating easy-to-interpret numerical representations of over 500 amino acid residues to enhance protein modeling and understanding of their activity.
- The proposed scales improve upon previous methods and show comparable or better performance in classifying G-protein coupled receptors than other existing scale sets.
- The research also examines the relationship between numerical indices and mutation patterns, emphasizing the need for balance between interpretability and relevance to protein structure conservation when developing custom scales.