Synthesis of Extracellular L-lysine-α-oxidase along with Degrading Enzymes by cf. Rifai VKM F-4268D: Role in Biocontrol and Systemic Plant Resistance.

When cultivating on wheat bran or deactivated fungal mycelium as a model of "natural growth", the ability of to synthesize extracellular L-lysine-α-oxidase (LysO) simultaneously with cell-wall-degrading enzymes (proteases, xylanase, glucanases, chitinases, etc.), responsible for mycoparasitism, was shown. LysO, in turn, causes the formation of HO and pipecolic acid.

Mechanism of Multi-Stage Degradation in Hot Bitumen of Micronized Powder Elastomeric Modifiers from Worn-Out Tire's Rubber.

For the first time, by atomic force microscopy (AFM) methods, micro- and nanofragments of micronized powder elastomeric modifier (PEM) formed at the short-term (3 min at 160 °C) interaction of PEM with hot bitumen have been demonstrated. It is the technology of high-temperature shear-induced grinding of a worn-out tire's crumb rubber or its co-grinding with styrene-butadiene-styrene (SBS) block copolymer which provides the creation of the PEM structure inclined to rapid degradation in hot bitumen. The formation just after the preparation process of a new structure of a modified binder, more resistant to external effects, is supported by the data of rheological tests.

Ultra-Dispersed Powders Produced by High-Temperature Shear-Induced Grinding of Worn-Out Tire and Products of Their Interaction with Hot Bitumen.

Structural features of crumb rubber (CR) particles obtained by grinding on rollers and ultra-disperse powder elastomeric modifiers (PEM) obtained by high-temperature shear-induced grinding (HTSG) of CR or co-grinding with butadiene styrene thermoplastic elastomer (SBS) have been studied by electron and optical microscopy methods. Samples of modified bitumen were obtained at different mixing times (1-40 min) in a wide temperature range (120-180 °C). The products of interaction of PEM with hot bitumen precipitated on filters when washed with solvent from modified bitumen (MB) were studied by scanning electron microscopy (SEM).