Publications by authors named "Alexander Balkin"

Mapping transcription start sites and determining their activity remain a challenging task even for well-studied organisms. Here, we present Cappable-seq RNA sequencing data of K-12 MG1655 after treatment with three antibiotics with various spectra of action that may expand the range of mapped transcription start sites for this model organism.

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The influence of microalgae on the formation of associated prokaryotic assemblages in halophilic microbial communities is currently underestimated. The aim of this study was to characterize shifts in prokaryotic assemblages of halophilic microalgae upon their transition to laboratory cultivation. Monoalgal cultures belonging to the classes Chlorodendrophyceae, Bacillariophyceae, Trebouxiophyceae, and Chlorophyceae were isolated from habitats with intermediate salinity, about 100 g/L, nearby Elton Lake (Russia).

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The quick-to-court (qtc) gene is expressed in both males and females but affects only the mating behavior of males, probably due to the different composition of isoforms between the sexes. We tested this hypothesis and examined the sex-specific expression of qtc transcripts in the tissues of male and female Oregon-R flies. It was found that some qtc transcripts, such as qtc-RM and qtc-RN, are testis-specific, while others like qtc-RH are found in ovaries but absent in testes.

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A rhizosphere strain, Achromobacter insolitus LCu2, was isolated from alfalfa (Medicago sativa L.) roots. It was able to degrade of 50% glyphosate as the sole phosphorus source, and was found resistant to 10 mM copper (II) chloride, and 5 mM glyphosate-copper complexes.

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Background: Cave biotopes are characterized by stable low temperatures, high humidity, and scarcity of organic substrates. Despite the harsh oligotrophic conditions, they are often inhabited by rich microbial communities. Abundant fouling with a wide range of morphology and coloration of colonies covers the walls of the Shulgan-Tash cave in the Southern Urals.

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Centrohelids (Haptista: Centroplasthelida) are axopodial protists with a remarkable diversity of external siliceous scale morphologies. It is believed that the last common ancestor of centrohelids had a double layer of siliceous scales composed of plate scales closer to a cell surface and spine scales radiating outwards. The characteristic morphotype of spine scales with a heart-shaped base was once believed to be a unique feature of the genus Choanocystis, as it was defined by Siemensma and Roijackers (1988).

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The bacterial pathogen , which causes enteritis, has a broad host range and extensive environmental longevity. In water and soil, Salmonella interacts with protozoa and multiplies inside their phagosomes. Although this relationship resembles that between and mammalian phagocytes, the interaction mechanisms and bacterial genes involved are unclear.

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Snow mold is a severe plant disease caused by psychrophilic or psychrotolerant fungi, of which species are the most harmful. A clear understanding of biology has many gaps; the pathocomplex and its dynamic are poorly characterized, virulence factors are unknown, genome sequences are not available, and the criteria of plant snow mold resistance are not elucidated. Our study aimed to identify comprehensive characteristics of a local community of snow mold-causing species colonizing a particular crop culture.

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is an ubiquitous pathogen throughout the world causing gastroenteritis in humans and animals. Survival of pathogenic bacteria in the external environment may be associated with the ability to overcome the stress caused by starvation. The bacterial response to starvation is well understood in laboratory cultures with a sufficiently high cell density.

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In enteric bacteria, DNA supercoiling is highly responsive to environmental conditions. Host specific features of environment serve as cues for the expression of genes required for colonization of host niches via changing supercoiling [1]. It has been shown that substitution at position 87 of GyrA of str.

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Plant growth-promoting rhizobacteria (PGPR) improve plant productivity and stress resistance. The mechanisms involved in plant-microbe interactions include the modulation of plant hormone status. The sp.

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Article Synopsis
  • - Ciliates, particularly Paramecium, are common aquatic protists that feed on bacteria and can be easily grown in labs, but their associated prokaryotic communities haven't been studied much.
  • - Researchers used high-throughput sequencing of the 16S rRNA gene to analyze the microbiomes of various Paramecium strains from different locations, looking for biases in HTS results based on DNA library preparation.
  • - The study found significant differences in the prokaryotic composition and bacterial diversity of the microbiomes related to ciliates and their environments, with some strains hosting potentially harmful bacteria.
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Article Synopsis
  • - This study investigates the link between dental caries and bronchial asthma severity in children, focusing on differences in the oral microbiome of asthmatic kids with and without dental caries using 16S rDNA sequencing.
  • - Dental plaque samples were taken from children and analyzed, revealing no significant differences in the overall bacterial community composition between both groups, although certain species were more prevalent in specific cases.
  • - The findings showed a higher abundance of the genus Veillonella in caries-affected children, suggesting its role in dental caries development, while potential respiratory pathogens were found in the mouths of both asthmatic groups.
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