Publications by authors named "Alex N T Johnson"
Multiplexed proteomics has become a powerful tool for investigating biological systems. Using balancer-peptide conjugates (e.g.
View Article and Find Full Text PDFOmpA controls order in the outer membrane and shares the mechanical load.
Proc Natl Acad Sci U S A
December 2024
Article Synopsis
- OmpA is an important outer membrane protein that influences bacterial virulence, adhesion, and membrane integrity, but its exact role has been unclear for over 50 years.
- This study reveals that OmpA plays a key role in organizing the outer membrane protein structure and connects it to the cell wall, helping to maintain the bacteria's protective barrier.
- The research shows that both parts of OmpA—its β-barrel domain and cell wall-binding domain—are essential for strengthening the bacterial envelope, making it more resilient and crucial for bacterial survival.
Extracellular-signal-regulated kinase (ERK) signaling controls development and homeostasis and is genetically deregulated in human diseases, including neurocognitive disorders and cancers. Although the list of ERK functions is vast and steadily growing, the full spectrum of processes controlled by any specific ERK activation event remains unknown. Here, we show how ERK functions can be systematically identified using targeted perturbations and global readouts of ERK activation.
View Article and Find Full Text PDFProtein turnover is critical for proteostasis, but turnover quantification is challenging, and even in well-studied E. coli, proteome-wide measurements remain scarce. Here, we quantify the turnover rates of ~3200 E.
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