Targeted deletion of Pf prophages from diverse isolates has differential impacts on quorum sensing and virulence traits.

is an opportunistic bacterial pathogen that commonly causes medical hardware, wound, and respiratory infections. Temperate filamentous Pf phages that infect impact numerous virulence phenotypes. Most work on Pf phages has focused on Pf4 and its host PAO1.

Neuronal alterations in AKT isotype expression in schizophrenia.

Schizophrenia is characterized by substantial alterations in brain function, and previous studies suggest insulin signaling pathways, particularly involving AKT, are implicated in the pathophysiology of the disorder. This study demonstrates elevated mRNA expression of AKT1-3 in neurons from schizophrenia subjects, contrary to unchanged or diminished total AKT protein expression reported in previous postmortem studies, suggesting a potential decoupling of transcript and protein levels. Sex-specific differential AKT activity was observed, indicating divergent roles in males and females with schizophrenia.

Targeted deletion of Pf prophages from diverse isolates impacts quorum sensing and virulence traits.

is an opportunistic bacterial pathogen that commonly causes medical hardware, wound, and respiratory infections. Temperate filamentous Pf phages that infect impact numerous bacterial virulence phenotypes. Most work on Pf phages has focused on strain Pf4 and its host PAO1.

Computational approaches to identify sites of phosphorylation.

Due to their oftentimes ambiguous nature, phosphopeptide positional isomers can present challenges in bottom-up mass spectrometry-based workflows as search engine scores alone are often not enough to confidently distinguish them. Additional scoring algorithms can remedy this by providing confidence metrics in addition to these search results, reducing ambiguity. Here we describe challenges to interpreting phosphoproteomics data and review several different approaches to determine sites of phosphorylation for both data-dependent and data-independent acquisition-based workflows.

Polyamines and linear DNA mediate bacterial threat assessment of bacteriophage infection.

Monitoring the extracellular environment for danger signals is a critical aspect of cellular survival. However, the danger signals released by dying bacteria and the mechanisms bacteria use for threat assessment remain largely unexplored. Here we show that lysis of cells releases polyamines that are subsequently taken up by surviving cells via a mechanism that relies on Gac/Rsm signaling.

The effect of trunnionosis on the risk of re-revision following femoral head exchange in hip arthroplasty.

Background: Fretting corrosion at modular junctions contributes to arthroplasty failure. Currently, no evidence-based guidelines are available regarding the acceptable level of trunnion corrosion that can occur . We aimed to examine the relationship between trunnion corrosion and risk of re-revision to assist surgeons with intraoperative decision making.

The active kinome: The modern view of how active protein kinase networks fit in biological research.

Biological regulatory networks are dynamic, intertwined, and complex systems making them challenging to study. While quantitative measurements of transcripts and proteins are key to investigate the state of a biological system, they do not inform the "active" state of regulatory networks. In consideration of that fact, "functional" proteomics assessments are needed to decipher active regulatory processes.