SARS-CoV-2 Rapid Antigen Test Based on a New Anti-Nucleocapsid Protein Monoclonal Antibody: Development and Real-Time Validation.

SARS-CoV-2 diagnostic tests have become an important tool for pandemic control. Among the alternatives for COVID-19 diagnosis, antigen rapid diagnostic tests (Ag-RDT) are very convenient and widely used. However, as SARS-CoV-2 variants may continuously emerge, the replacement of tests and reagents may be required to maintain the sensitivity of Ag-RDTs.

There might be more to syntactic bootstrapping than being pragmatic: A look at grammatical person and prosody in naturalistic child-directed speech.

In 'Being pragmatic about syntactic bootstrapping', Hacquard (2022) argues that abstract syntax is useful for word learning, but that an additional cue, pragmatics, is both necessary and available for young children during the first steps of language acquisition. She focuses on modals and attitude verbs, where the physical context seems particularly impoverished as the sole basis for deriving meanings, and thus where linguistic cues may be particularly helpful. She convincingly shows how pragmatic and syntactic cues could be combined to help young language learners learn and infer the possible meanings of attitude verbs such as "think", "know" or "want".

"The tiger is hitting! the duck too!" 3-year-olds can use prosodic information to constrain their interpretation of ellipsis.

This work aims to investigate French children's ability to use phrasal boundaries for disambiguation of a type of ambiguity not yet studied, namely stripping sentences versus simple transitive sentences. We used stripping sentences such as "[Le tigre tape]! [Le canard aussi]!" ("[The tiger is hitting]! [The duck too]!", in which both the tiger and the duck are hitting), which, without the prosodic information, would be ambiguous with a transitive sentence such as "[Le tigre] [tape le canard aussi]!" ("[The tiger] [is hitting the duck too]!", in which the tiger is hitting the duck). We presented 3-to-4-year-olds and 28-month-olds with one of the two types of sentence above, while they watched two videos side-by-side on a screen: one depicting the transitive interpretation of the sentences, and another depicting the stripping interpretation.

"Look! It is not a bamoule!": 18- and 24-month-olds can use negative sentences to constrain their interpretation of novel word meanings.

Two word-learning experiments were conducted to investigate the understanding of negative sentences in 18- and 24-month-old children. In Experiment 1, after learning that bamoule means "penguin" and pirdaling means "cartwheeling," 18-month-olds (n = 48) increased their looking times when listening to negative sentences rendered false by their visual context ("Look! It is not a bamoule!" while watching a video showing a penguin cartwheeling); however, they did not change their looking behavior when negative sentences were rendered true by their context ("Look! It is not pirdaling!" while watching a penguin spinning). In Experiment 2, 24-month-olds (n = 48) were first exposed to a teaching phase in which they saw a new cartoon character on a television (e.

Toddlers exploit referential and syntactic cues to flexibly adapt their interpretation of novel verb meanings.

Because linguistic communication is often noisy and uncertain, adults flexibly rely on different information sources during sentence processing. We tested whether toddlers engage in a similar process and how that process interacts with verb learning. Across two experiments, we presented French 28-month-olds with right-dislocated sentences featuring a novel verb ("He is VERBing, the boy"), where a clear prosodic boundary after the verb indicates that the sentence is intransitive (such that the NP "the boy" is coreferential with the pronoun "he" and the sentence means "The boy is VERBing").

Familiar words can serve as a semantic seed for syntactic bootstrapping.

Young children can exploit the syntactic context of a novel word to narrow down its probable meaning. But how do they learn which contexts are linked to which semantic features in the first place? We investigate if 3- to 4-year-old children (n = 60) can learn about a syntactic context from tracking its use with only a few familiar words. After watching a 5-min training video in which a novel function word (i.

Use of the Facial Artery for Free Functioning Muscle Transfers: An Alternative Pedicle for Salvage in Brachial Plexus Lesions with Vascular Injuries.

Free functional muscle transfer (FFMT) is a salvage procedure recommended in cases of brachial plexus injury with late presentations or failures of primary nerve reconstruction. The workhorse for most authors is the gracilis, and the most common indication is the restoration of elbow flexion. For successful revascularization of the muscle, donor vessels must be in proximity of the site of the muscle fixation and allow direct coaptation to a donor nerve, ideally without the use of nerve grafts.

Effects of temperature changes in the transcriptional profile of the emerging fish pathogen Francisella noatunensis subsp. orientalis.

One of the major challenges in Nile tilapia (Oreochromis niloticus L.) farming is the occurrence of bacterial infections, and the Francisella noatunensis subsp. orientalis (FNO) is an important pathogen that has emerged in last decades.

Studying the Real-Time Interpretation of Novel Noun and Verb Meanings in Young Children.

Decades of research show that children rely on the linguistic context in which novel words occur to infer their meanings. However, because learning in these studies was assessed after children had heard numerous occurrences of a novel word in informative linguistic contexts, it is impossible to determine how much exposure would be needed for a child to learn from such information. This study investigated the speed with which French 20-month-olds and 3-to-4-year-olds exploit function words to determine the syntactic category of novel words and therefore infer their meanings.

Complete genome sequencing of sixteen Francisella noatunensis subsp. orientalis isolates: A genomic approach for molecular characterization and spread dynamics of this clonal population.

Francisella noatunensis subsp. orientalis (FNO) is an important emerging pathogen associated with disease outbreaks in farm-raised Nile tilapia. FNO genetic diversity using PCR-based typing, no intra-species discrimination was achieved among isolates/strains from different countries, thus demonstrating a clonal behaviour pattern.

Three- to Four-Year-Old Children Rapidly Adapt Their Predictions and Use Them to Learn Novel Word Meanings.

Adults create and update predictions about what speakers will say next. This study asks whether prediction can drive language acquisition, by testing whether 3- to 4-year-old children (n = 45) adapt to recent information when learning novel words. The study used a syntactic context which can precede both nouns and verbs to manipulate children's predictions about what syntactic category will follow.

Phrasal prosody constrains syntactic analysis in toddlers.

This study examined whether phrasal prosody can impact toddlers' syntactic analysis. French noun-verb homophones were used to create locally ambiguous test sentences (e.g.

Scalar Implicatures: The Psychological Reality of Scales.

Scalar implicatures, the phenomena where a sentence like "The pianist played Mozart sonatas" is interpreted, as "The pianist did play Mozart sonatas" have been given two different analyses. Neo-Griceans (NG) claim that this interpretation is based on lexical scales (e.g.

Ambiguous function words do not prevent 18-month-olds from building accurate syntactic category expectations: An ERP study.

To comprehend language, listeners need to encode the relationship between words within sentences. This entails categorizing words into their appropriate word classes. Function words, consistently preceding words from specific categories (e.

English-speaking preschoolers can use phrasal prosody for syntactic parsing.

This study tested American preschoolers' ability to use phrasal prosody to constrain their syntactic analysis of locally ambiguous sentences containing noun/verb homophones (e.g., [The baby flies] [hide in the shadows] vs [The baby] [flies his kite], brackets indicate prosodic boundaries).

A MLST Clade 2 Clostridium difficile strain with a variant TcdB induces severe inflammatory and oxidative response associated with mucosal disruption.

The epidemiology of Clostridium difficile infections is highly dynamic as new strains continue to emerge worldwide. Here we present a detailed analysis of a new C. difficile strain (ICC-45) recovered from a cancer patient in Brazil that died from severe diarrhea.

Complete genome sequence of Streptococcus agalactiae strain GBS85147 serotype of type Ia isolated from human oropharynx.

Streptococcus agalactiae, also referred to as Group B Streptococcus, is a frequent resident of the rectovaginal tract in humans, and a major cause of neonatal infection. The pathogen can also infect adults with underlying disease, particularly the elderly and immunocompromised ones. In addition, S.

Whole-genome optical mapping reveals a mis-assembly between two rRNA operons of Corynebacterium pseudotuberculosis strain 1002.

Background: Studies have detected mis-assemblies in genomes of the species Corynebacterium pseudotuberculosis. These new discover have been possible due to the evolution of the Next-Generation Sequencing platforms, which have provided sequencing with accuracy and reduced costs. In addition, the improving of techniques for construction of high accuracy genomic maps, for example, Whole-genome mapping (WGM) (OpGen Inc), have allow high-resolution assembly that can detect large rearrangements.

Complete genome sequences of Francisella noatunensis subsp. orientalis strains FNO12, FNO24 and FNO190: a fish pathogen with genomic clonal behavior.

The genus Francisella is composed of Gram-negative, pleomorphic, strictly aerobic and non-motile bacteria, which are capable of infecting a variety of terrestrial and aquatic animals, among which Francisella noatunensis subsp. orientalis stands out as the causative agent of pyogranulomatous and granulomatous infections in fish. Accordingly, F.

Genome-Wide Screening of mRNA Expression in Leprosy Patients.

Leprosy, an infectious disease caused by Mycobacterium leprae, affects millions of people worldwide. However, little is known regarding its molecular pathophysiological mechanisms. In this study, a comprehensive assessment of human mRNA was performed on leprosy skin lesions by using DNA chip microarrays, which included the entire spectrum of the disease along with its reactional states.

Gene expression patterns through oral squamous cell carcinoma development: PD-L1 expression in primary tumor and circulating tumor cells.

Oral squamous cell carcinoma (OSCC) is the most common tumor of the oral cavity and has been associated with poor prognosis. Scarce prognostic markers are available for guiding treatment and/or sub-classifying patients. This study aims to identify biomarkers by searching for genes whose expression is increased or decreased during tumor progression (through T1 to T4 stages).