Publications by authors named "Aletta Kiss"
Article Synopsis
- 3D cell cultures, particularly patient-derived organoids (PDOs), are revolutionizing research on pancreatic functions and diseases, tackling major challenges like pancreatitis.* -
- The article provides detailed protocols for creating and maintaining pancreatic PDOs, including techniques for polarity switching, and includes a troubleshooting guide.* -
- By outlining both the processes and cost considerations, the authors encourage a shift from using rodent models to primary human cells, supporting efforts to improve clinical research reliability.*
Epithelial ion and fluid secretion determine the physiological functions of a broad range of organs, such as the lung, liver, or pancreas. The molecular mechanism of pancreatic ion secretion is challenging to investigate due to the limited access to functional human ductal epithelia. Patient-derived organoids may overcome these limitations, however direct accessibility of the apical membrane is not solved.
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- * There are currently no targeted treatments for either RAP or early CP, making it important to explore the underlying cell interactions that lead to disease progression.
- * This study found that inhibiting Orai1, a key signaling mechanism linked to calcium overload in pancreatic cells, can halt the progression of RAP and early CP, suggesting that targeting Orai1 might help prevent severe stages of CP.
Alcoholic pancreatitis and hepatitis are frequent, potentially lethal diseases with limited treatment options. Our previous study reported that the expression of CFTR Cl channel is impaired by ethanol in pancreatic ductal cells leading to more severe alcohol-induced pancreatitis. In addition to determining epithelial ion secretion, CFTR has multiple interactions with other proteins, which may influence intracellular Ca signaling.
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