Publications by authors named "Alessandro Rossetta"
Article Synopsis
- Ribonucleoprotein (RNP) condensates create distinct liquid phases that make it hard to study their functions versus the surrounding environment.
- Researchers implemented fluorescence lifetime imaging microscopy (FLIM) alongside phasor plot filtering to better differentiate these condensates and assess protein interactions using FLIM-Förster resonance energy transfer (FRET).
- They found that interactions among decapping complex subunits in P-bodies change based on conditions, particularly observing altered interactions during oxidative stress, highlighting the dynamic nature of these protein networks in live cells.
Article Synopsis
- Pancreatic cancer is hard to treat because it's often diagnosed at an advanced stage, lacks early symptoms, and has few reliable biomarkers for detection.
- *Recent research shows that interactions between cancer and immune cells in both the tumor micro-environment and distant areas of the body (macro-environment) play a crucial role in tumor growth and immune response.
- *Understanding how these environments interact may lead to new early detection methods and therapies for pancreatic cancer through identifying molecular targets and improving diagnostic biomarkers.
The presence of plastic fragments in aquatic environments, particularly at the micro- and nano-scale, has become a significant global concern. However, current detection methods are limited in their ability to reveal the presence of such particles in liquid samples. In this study, we propose the use of a fluorescence lifetime analysis system for the detection of micro- and nanoplastics in water.
View Article and Find Full Text PDFFluorescence laser-scanning microscopy (LSM) is experiencing a revolution thanks to new single-photon (SP) array detectors, which give access to an entirely new set of single-photon information. Together with the blooming of new SP LSM techniques and the development of tailored SP array detectors, there is a growing need for (i) DAQ systems capable of handling the high-throughput and high-resolution photon information generated by these detectors, and (ii) incorporating these DAQ protocols in existing fluorescence LSMs. We developed an open-source, low-cost, multi-channel time-tagging module (TTM) based on a field-programmable gate array that can tag in parallel multiple single-photon events, with 30 ps precision, and multiple synchronisation events, with 4 ns precision.
View Article and Find Full Text PDFFluorescence lifetime imaging microscopy (FLIM) and spectral imaging are two broadly applied methods for increasing dimensionality in microscopy. However, their combination is typically inefficient and slow in terms of acquisition and processing. By integrating technological and computational advances, we developed a robust and unbiased spectral FLIM (S-FLIM) system.
View Article and Find Full Text PDFLipofectamine reagents are widely accepted as "gold-standard" for the safe delivery of exogenous DNA or RNA into cells. Despite this, a satisfactory mechanism-based explanation of their superior efficacy has remained mostly elusive thus far. Here we apply a straightforward combination of live cell imaging, single-particle tracking microscopy, and quantitative transfection-efficiency assays on live cells to unveil the intracellular trafficking mechanism of Lipofectamine/DNA complexes.
View Article and Find Full Text PDF