Predation cues induce predator specific changes in olfactory neurons encoding defensive responses in agile frog tadpoles.

Although behavioural defensive responses have been recorded several times in both laboratory and natural habitats, their neural mechanisms have seldom been investigated. To explore how chemical, water-borne cues are conveyed to the forebrain and instruct behavioural responses in anuran larvae, we conditioned newly hatched agile frog tadpoles using predator olfactory cues, specifically either native odonate larvae or alien crayfish kairomones. We expected chronic treatments to influence the basal neuronal activity of the tadpoles' mitral cells and alter their sensory neuronal connections, thereby impacting information processing.

Multimodal Cues Do Not Improve Predator Recognition in Green Toad Tadpoles.

The anti-predator behaviour of green toad () tadpoles was investigated by exposing them to only the visual or chemical cues, or a combination of both, of a native predator, southern hawker . We collected green toad egg strings in the field and tadpoles did not receive any predatory stimulus before the onset of the experiment. To manipulate chemical and visual cues independently, dragonfly larvae were caged inside a transparent plastic container, while chemical cues (odour of tadpole-fed dragonfly larvae) were injected into the surrounding arena.

Anti-predator behavioral responses of Italian agile frog tadpoles (Rana latastei) exposed to microplastics.

Microplastics (MPs) are nowadays abundant, persistent, and ubiquitous in the environment, representing a new threat for terrestrial, marine, and freshwater ecosystems. Although anuran populations and species are globally declining, the effect of MP exposure on this taxon has been poorly investigated. With the aim of assessing the effects of microplastic exposure on the defensive responses of Italian agile frog (Rana latastei) tadpoles, we exposed them to three different concentrations (1, 7, and 50 mg L) of a mixture of plastic polymers (HPDE, PVC, PS, and PES) for 2 weeks.

Effects of predation risk on the sensory asymmetries and defensive strategies of Bufotes balearicus tadpoles.

Lateralization consists of the differential use of bilateral organs or limbs and is well described in many taxa and in several contexts. Common ecological frameworks where it can be observed are foraging and predatory ones, with benefits related to both visual and auditory lateralization such as faster response or increasing neural processing ability. Anuran amphibians are considered relevant models for investigating lateralization, due to their great ecological variety and the possibility of easily being raised under laboratory conditions.

Microplastic contamination in terrestrial ecosystems: A study using barn owl (Tyto alba) pellets.

Microplastics (MPs) are recognised as an emerging environmental problem that needs to be carefully monitored. So far, MPs have been widely recorded in marine and freshwater ecosystems. Still, few studies have focused on MP occurrence in terrestrial ecosystems, although soils are suspected to be one of the main MP reservoirs.

Differential effects of microplastic exposure on anuran tadpoles: A still underrated threat to amphibian conservation?

Microplastics (MPs) have been reported to threaten a wide variety of terrestrial, marine, and freshwater organisms. However, knowledge about the effects of MPs on anuran amphibians, one of the most threatened taxa worldwide, is still limited. To assess the effects of MPs on the growth and survival of the Italian agile frog (Rana latastei) and green toad (Bufotes balearicus), we exposed tadpoles to three different concentrations (1, 7, and 50 mg L) of an environmental relevant mixture of microplastics (HPDE, PVC, PS and PES), recording data on their activity level, weight and mortality rates.

Contextual behavioural plasticity in Italian agile frog (Rana latastei) tadpoles exposed to native and alien predator cues.

Predation is a strong driver for the evolution of prey behaviour. To properly assess the actual risk of predation, anuran tadpoles mostly rely on water-borne chemical cues, and their ability to evaluate environmental information is even more crucial when potential predators consist of unknown alien species. Behavioural plasticity - that is, the capacity to express changes in behaviour in response to different environmental stimuli - is crucial to cope with predation risk.

Long-term impact of agricultural practices on the diversity of small mammal communities: a case study based on owl pellets.

Small mammals have been seldom used as indicators of biodiversity responses to environmental changes, probably because their long-term population trend in a given area is not easy to monitor. To assess the impact of agricultural intensification in a protected area of northern Italy, we compared the composition of its small mammal communities, as assessed in 1994-1995 and 2015-2016 by the analysis of owl pellets (N = 265 and 302, respectively), which provides an effective and affordable method for assessing changes in the diversity and structure of small mammal assemblages over time. We recorded a sharp reduction in the frequency of occurrence of shrews (Sorex spp.

Predation on Amphibians May Enhance Eurasian Otter Recovery in Southern Italy.

Mediterranean freshwaters undergo extreme seasonal variation in water flow, which, exacerbated by water withdrawal for agriculture or hydroelectric purposes, may affect fish communities and thus prey availability for semi-aquatic predators, such as Eurasian otter . To investigate the role played by food availability on the ongoing recovery of an otter population at the southernmost limit of its Italian range, we assessed otter diet by the analysis of 357 spraints collected from 2014 to 2017 on eight rivers, and both fish and amphibian availability by, respectively, electrofishing and visual encounter surveys. Fish and amphibians formed the bulk of otter diet, the latter resource contributing as much as fish to otter diet in spring.

Discrimination of group numerousness under predation risk in anuran tadpoles.

For social animals, group size discrimination may play a major role in setting the trade-off between the costs and benefits of membership. Several anuran tadpoles show different degrees of social aggregation when exposed to the risk of predation. Despite the importance of aggregative behaviour as an anti-predatory response, the mechanism underlying tadpole choice of the group to join to has not been sufficiently investigated.

XMRV and Public Health: The Retroviral Genome Is Not a Suitable Template for Diagnostic PCR, and Its Association with Myalgic Encephalomyelitis/Chronic Fatigue Syndrome Appears Unreliable.

A few years ago, a highly significant association between the xenotropic murine leukemia virus-related virus (XMRV) and myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS), a complex debilitating disease of poorly understood etiology and no definite treatment, was reported in , raising concern for public welfare. Successively, the failure to reproduce these findings, and the suspect that the diagnostic PCR was vitiated by laboratory contaminations, led to the retraction of the paper. Notwithstanding, XMRV continued to be the subject of researches and public debates.

Modelling the Distribution of Forest-Dependent Species in Human-Dominated Landscapes: Patterns for the Pine Marten in Intensively Cultivated Lowlands.

In recent years, the "forest-specialist" pine marten Martes martes has been reported to also occur also in largely fragmented, lowland landscapes of north-western Italy. The colonization of such an apparently unsuitable area provided the opportunity for investigating pine marten ecological requirements and predicting its potential south- and eastwards expansion. We collected available pine marten occurrence data in the flood plain of the River Po (N Italy) and relate them to 11 environmental variables by developing nine Species Distribution Models.

Detectability of the Eurasian otter by standard surveys: an approach using marking intensity to estimate false negative rates.

False negative detections may bias the surveys for rare species and reduce the reliability of models based on the proportion of occupied patches. We assessed the detectability of the Eurasian otter Lutra lutra through the standard survey method by analysing the detection history of 28 sampling stretches surveyed monthly between March 2001 and January 2003. Each survey negative for otter spraints was considered as a false negative if the otter had been recorded in the previous and/or following month (respectively, cFN and FN).

Exploitation of food resources by the Eurasian badger (Meles meles) at the altitudinal limit of its alpine range (NW Italy).

Food availability has been suggested to be the main factor shaping the altitudinal limits of species distributions. We analyzed the badger (Meles meles) diet in the western Italian Alps and, particularly, at the altitudinal limit of its range, with the main aim of highlighting any reduction in earthworm availability with altitude which could act as a limiting factor for badgers. Earthworms were by far the main food resource of badgers, followed by fruit.

Red fox (Vulpes vulpes) cannibalistic behaviour and the prevalence of Trichinella britovi in NW Italian Alps.

Food habits of the red fox (Vulpes vulpes) were studied in the Aosta Valley region (NW Italian Alps) and were related to the prevalence of Trichinella infection in the red fox itself and in two Mustelid species (the stone marten (Martes foina) and the badger (Meles meles)). The search of Trichinella by the automatic digestion of muscles samples led us to determine a prevalence of 3.5+/-1.