In Vitro Antimicrobial Susceptibility of Mycobacterium massiliense Recovered from Wound Samples of Patients Submitted to Arthroscopic and Laparoscopic Surgeries.

Testing of rapidly growing species of mycobacteria (RGM) against antibacterial agents has been shown to have some clinical utility. This work establishes the MICs of seven antimicrobial agents following the guidelines set forth by the Clinical and Laboratory Standards Institute (CLSI) against eighteen isolates of Mycobacterium massiliense recovered from wound samples of patients submitted to minimally invasive surgery such as arthroscopy and laparoscopy. The isolates showed susceptibility to amikacin (MIC(90) = 4 μg/mL) and clarithromycin (MIC(90) < 1 μg/mL) but resistance to ciprofloxacin (MIC(90) > 16 μg/mL), doxycycline (MIC(90) > 32 μg/mL), sulfamethoxazole (MIC(90) > 128 μg/mL), and tobramycin (MIC(90) = 32 μg/mL), and intermediate profile to cefoxitin (MIC(90) = 64 μg/mL).

Acute immune response to Mycobacterium massiliense in C57BL/6 and BALB/c mice.

Mycobacterium massiliense is an environmental opportunistic pathogen that has been associated with soft tissue infection after minor surgery. We studied the acute immune response of C57BL/6 and BALB/c mice infected intravenously with 10(6) CFU of an M. massiliense strain isolated from a nosocomial infection in Brazil.

Emergence of nosocomial Mycobacterium massiliense infection in Goiás, Brazil.

A cluster of surgical site infection cases after arthroscopic and laparoscopic procedures occurred between 2005 and 2007 in Goiânia, in the central region of Brazil. Nontuberculous mycobacteria (NTM) were isolated from samples (exudates from cutaneous abscesses) from 18 patients of seven private hospitals. There were no reports of post-surgical arthroscopic and laparoscopic mycobacterial infections in Goiânia apart from this period.