Identification of Y-Chromosome Sequences in Turner Syndrome.

Objectives: To investigate the presence of Y-chromosome sequences and determine their frequency in patients with Turner syndrome.

Methods: The study included 23 patients with Turner syndrome from Brazil, who gave written informed consent for participating in the study. Cytogenetic analyses were performed in peripheral blood lymphocytes, with 100 metaphases per patient.

[Prevalence of Y-chromosome sequences and gonadoblastoma in Turner syndrome].

Objective:: To assess the prevalence of Y-chromosome sequences and gonadoblastoma in patients with Turner syndrome (TS) using molecular techniques.

Data Source:: A literature search was performed in Pubmed, limiting the period of time to the years 2005–2014 and using the descriptors: TS and Y sequences (n=26), and TS and Y-chromosome material (n=27). The inclusion criteria were: articles directly related to the subject and published in English or Portuguese.

[Characterization of family health teams and their work process].

This study aims to learn the characteristics of Family Health Strategy (FHS) teams from a Regional Health Coordination of RS, identifying its difficulties in the work process. It is a qualitative exploratory-descriptive study, with a questionnaire application. Results revealed a group of young females with recent professional education.

Solubilization of proteins from human lymph node tissue and two-dimensional gel storage.

In the present study, we compared six different solubilization buffers and optimized two-dimensional electrophoresis (2-DE) conditions for human lymph node proteins. In addition, we developed a simple protocol for 2-D gel storage. Efficient solubilization was obtained with lysis buffers containing (a) 8 M urea, 4% CHAPS (3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate), 40 mM Tris base, 65 mM DTT (dithiothreitol) and 0.

Expression, purification, and circular dichroism analysis of human CDK9.

The human cyclin-dependent kinase 9 (CDK9) protein was expressed in E. coli BL21 using the pET23a vector at 30 degrees C. Several milligrams of protein were purified from soluble fraction using ionic exchange and ATP-affinity chromatography.