Development and Evaluation of a Novel Taqman Real-Time PCR Assay for Rapid Detection of : Comparison of Assay Performance with a Conventional PCR Assay and Another Taqman Real-Time PCR Assay.

The objective of this study was to develop and validate a Taqman real-time PCR assay for the detection of (). Unique primers targeting the highly conserved house-keeping gene () were designed and the probe sequence was derived from a previously published microarray study. There was 100% agreement in the outcome between our assay and the other two published assays for detection.