Publications by authors named "Alena Blank-Giwojna"
RNA can bind within the major groove of purine-rich DNA via Hoogsteen base pairing and form a triple helical RNA-DNA structure that anchors the RNA to specific DNA sequences, thereby targeting RNA-associated regulatory proteins to distinct genomic sites. Here we present methods to analyze the potential of a given RNA to form triplexes in vitro and to validate these structures in vivo.
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- KHPS1, an antisense RNA, regulates the proto-oncogene SPHK1 by creating a triple-helical structure with DNA at the SPHK1 enhancer, promoting its expression.
- The triplex formation is crucial for recruiting transcription factors E2F1 and p300, which initiate transcription of an enhancer-derived RNA called eRNA-Sphk1.
- Deleting the triplex-forming sequence reduces SPHK1 expression and negatively affects cell migration and invasion, indicating the importance of this regulatory mechanism in cancer biology.