Publications by authors named "Aleksey Masharsky"
This study aimed to determine phenotypic and genotypic drug resistance patterns of strains from children with tuberculosis (TB) in China and Russia, two high-burden countries for multi/extensively-drug resistant (MDR/XDR) TB. Whole-genome sequencing data of isolates from China ( = 137) and Russia ( = 60) were analyzed for phylogenetic markers and drug-resistance mutations, followed by comparison with phenotypic susceptibility data. The Beijing genotype was detected in 126 Chinese and 50 Russian isolates.
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- The study focuses on the Mycobacterium tuberculosis Beijing 14717-15-cluster, which is a multidrug-resistant and hypervirulent strain found mainly in the Far Eastern region of Russia.
- Researchers collected and analyzed M. tuberculosis DNA from worldwide locations between 1996 and 2020, developing a PCR assay to identify specific mutations related to this deadly strain.
- Phylogenomic analysis confirmed the strain's prevalence in Asian Russia and highlighted unique mutations that could enhance its pathogenicity, suggesting the need for further research on these mutations' biological effects.
We performed synthesis of new nitrofuranyl amides and investigated their anti-TB activity and primary genetic response of mycobacteria through whole-genome sequencing (WGS) of spontaneous resistant mutants. The in vitro activity was assessed on reference strain H37Rv. The most active compound was used for in vitro selection of spontaneous resistant mutants.
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- - Preeclampsia (PE) is a critical pregnancy complication linked to high maternal and fetal mortality, highlighting the need for early diagnosis and treatment.
- - Researchers conducted targeted next-generation sequencing to analyze genetic variations in patients with PE compared to a control group, utilizing bioinformatics tools for assessment.
- - They identified several genetic markers associated with PE and one variant potentially protective against it, but further investigation is needed to fully understand the gene's role in the disease's development during pregnancy.