Publications by authors named "Aleksei V Sukhozhenko"
Cell surface display of Yarrowia lipolytica lipase Lip2p using the cell wall protein YlPir1p, its characterization, and application as a whole-cell biocatalyst.
Evgeniya Y Yuzbasheva Tigran V Yuzbashev Natalia I Perkovskaya Elizaveta B Mostova Tatiana V Vybornaya Aleksei V Sukhozhenko
Appl Biochem Biotechnol
April 2015
Article Synopsis
- The Yarrowia lipolytica lipase Lip2p was successfully displayed on yeast cells using a fusion with the YlPir1p protein, achieving a high hydrolytic activity of 11,900 U/g of dry weight.
- However, there was some leakage of the enzyme from the cell wall, with around 600,000 molecules of the recombinant enzyme present per cell, approaching the maximum limit.
- Despite the leakage, the surface-displayed lipase showed improved stability at high temperatures and in organic solvents, and was effectively utilized in recycling biodiesel synthesis, achieving significant yields of methyl esters in multiple reaction cycles.