Application of Scanning Electron Microscopy in the observation of dentin-adhesive interface.

The objective of this study was observation of the adhesive interface on original tooth samples, as well as their epoxy replicas, under SEM. A light-cure flowable composite was incrementally placed and light-polymerized in previously prepared cylindrical dentinal cavities on the buccal surfaces of extracted human third molars. After finishing procedures, impressions of the composite/dentin margin were made using polyvinylsiloxane in order to obtain accurate epoxy replicas for SEM analysis.