Optimization of Eco-Friendly Extraction of Bioactive Compounds from Mentha spicata L. Using Ultrasound-Assisted Extraction Combined with Choline Chloride-Based Deep Eutectic Solvents.

Three choline chloride (ChCl)-based deep eutectic solvents (DESs) as a new type of green solvents were used for the ultrasound-assisted extraction (UAE) of bioactive compounds from Mentha spicata L. DES containing ChCl and malonic acid (MalA) was selected as the most promising, providing a more effective extraction of antioxidants from spearmint. Response surface methodology (RSM) and a Box-Behnken design (BBD) with three variables, ChCl:MalA molar ratio, water content (WC) in DES, and extraction time (t), were implemented for optimizing the extraction conditions.

Polyphenols from Bee Pollen: Structure, Absorption, Metabolism and Biological Activity.

Bee pollen constitutes a natural source of antioxidants such as phenolic acids and flavonoids, which are responsible for its biological activity. Research has indicated the correlation between dietary polyphenols and cardioprotective, hepatoprotective, anti-inflammatory, antibacterial, anticancerogenic, immunostimulating, antianaemic effects, as well as their beneficial influence on osseous tissue. The beneficial effects of bee pollen on health result from the presence of phenolic acids and flavonoids which possess anti-inflammatory properties, phytosterol and linolenic acid which play an anticancerogenic role, and polysaccharides which stimulate immunological activity.

Caffeic Acid phenethyl ester and ethanol extract of propolis induce the complementary cytotoxic effect on triple-negative breast cancer cell lines.

Chemotherapy of breast cancer could be improved by bioactive natural substances, which may potentially sensitize the carcinoma cells' susceptibility to drugs. Numerous phytochemicals, including propolis, have been reported to interfere with the viability of carcinoma cells. We evaluated the in vitro cytotoxic activity of ethanol extract of propolis (EEP) and its derivative caffeic acid phenethyl ester (CAPE) towards two triple-negative breast cancer (TNBC) cell lines, MDA-MB-231 and Hs578T, by implementation of the MTT and lactate dehydrogenase (LDH) assays.

[The role of nicotine as a modulator of the activity of acid phosphotase and cathepsin D and L in the liver and kidney of mice].

To our knowledge, no reports on the impact of nicotine on the enzyme activity of the lysosomal system have been published yet. The study which is reported here is probably one of the first analyses of the models of lysosomal hydrolases of the liver and kidney of experimental mice which were exposed to various doses of nicotine. The aim of our experimental study was to analyze the impact of nicotine administered for 4 or 8 days in the dosage of 12 and 20 mg/kg body weight, respectively, on the activity of acid phosphatase and cathepsins D and L, i.