Houtt. Transformed Hairy Root Cultures as an Effective Platform for Producing Phenolic Compounds with Strong Bactericidal Properties.

Houtt. is the source of various phenolic compounds: phenolic acids, flawan-3-ols, and stilbenes, with a broad range of biological activity. The rhizome (underground organ of these plants) is abundant in secondary metabolites but, in natural conditions, may accumulate various toxic substances (such as heavy metals) from the soil.

The Substantial Role of Cell and Nanoparticle Surface Properties in the Antibacterial Potential of Spherical Silver Nanoparticles.

Purpose: Although it is well known that the size, shape, and surface chemistry affect the biological potential of silver nanoparticles (AgNPs), the published studies that have considered the influence of AgNP surface on antibacterial activity have not provided conclusive results. This is the first study whose objective was to determine the significance of the surface net charge of AgNPs on their antibacterial potential, attraction to bacterial cells, and cell envelope disruption, considering differences in bacterial surface properties.

Methods: We evaluated five commercial AgNP colloids with identical size and shape but different surface ligands.

Recent Progress in Laser Powder Bed Fusions Processes of Advanced High-Strength Steels.

This review is focused on the perspectives of the application of Advanced High Strength Steels (AHSSs) in the field of additive technologies directed at the laser powder bed fusion/selective laser melting processes. In principle, AHSSs require significant attention due to their promising mechanical properties for usage in the automotive industry towards reducing the weight of vehicles. Although additive manufacturing represents a promising perspective towards expanding the industrialization of AHSSs in a wider area of their applications, they have not been sufficiently investigated concerning their usage in LPBF/SLM processes.

Effect of Agitation and Temporary Immersion on Growth and Synthesis of Antibacterial Phenolic Compounds in Genus .

Sundews ( sp.) are the source of biologically active secondary metabolites: phenolic acids, flavonoids, and 1,4-naphtoquinones. Because obtaining them from the natural environment is impossible (rare and endangered species), in this study modifications of traditional tissue cultures grown in solid medium (SM), such as agitated cultures (ACs) (cultures in liquid medium with rotary shaking) and temporary immersion bioreactors Plantform (TIB), were used for multiplication of four sundew species: , , , and , with simultaneously effective synthesis of biologically active phenolic compounds.

Genetic Loci of Plant Pathogenic IPO 2222 Expressed in Contact with Weed-Host Bittersweet Nightshade ( L.) Plants.

, belonging to the Soft Rot , are aggressive necrotrophs, exhibiting both a wide geographic distribution and a wide host range that includes many angiosperm orders, both dicot and monocot plants, cultivated under all climatic conditions. Little is known about the infection strategies employs to infect hosts other than potato ( L.).

Immunomodulatory and antibacterial effect of red wine concentrate rich in a natural complex of polyphenols under diabetes mellitus.

Changes in immunocompetent cells influence the course of diabetes mellitus and contribute to its complications. Thus, correction of diabetes-induced immune system disorders is vital for normalizing the state of the organism. Red wine polyphenols due to their biological activities could be considered a potential remedy for correcting diabetes.

Biotechnological strategies for controlled accumulation of flavones in hairy root culture of Scutellaria lateriflora L.

Accumulation of medicinally important flavones and acteoside was evaluated in Scutellaria lateriflora hairy root cultures subjected to different experimental strategies - feeding with precursors of phenolics biosynthesis (phenylalanine, cinnamic acid, and sodium cinnamate), addition of elicitors (chitosan, jasmonic acid) and Amberlite XAD-4 and XAD-7 resins and permeabilization with dimethyl sulfoxide (DMSO) and methanol. The production profile of S. lateriflora cultures changed under the influence of the applied strategies.

In Vitro and In Vivo Studies of Antibacterial Coatings on Titanium Alloy Implants for Veterinary Application.

The aim of this work was the evaluation of biological properties of hybrid coatings modified with Ag, Cu, and Zn nanoparticles (NPs) applied on TPLO medical implants by the sol-gel process. The implant coatings enriched with various concentrations of metallic NPs were investigated in the in vitro bactericidal efficacy tests against Gram+ and Gram- bacteria and pathogenic yeast. Next, the designed materials were tested on human osteosarcoma cell lines.

Controlling the Thermal Stability of a Bainitic Structure by Alloy Design and Isothermal Heat Treatment.

The aim of this work was to develop a novel bainitic steel that will be specifically dedicated to achieving a high degree of refinement (nano- or submicron scale) along with increased thermal stability of the structure at elevated temperatures. The material was characterized by improved in-use properties, expressed as the thermal stability of the structure, compared to nanocrystalline bainitic steels with a limited fraction of carbide precipitations. Assumed criteria for the expected low martensite start temperature, bainitic hardenability level, and thermal stability are specified.

Exposure of Keratinocytes to in the Context of Atopic Induces Changes in the Surface Glycosylation Pattern of Small Extracellular Vesicles to Enhance Their Propensity to Interact With Inhibitory Siglec Receptors.

infection is a potential complication in the individuals with atopic dermatitis (AD) and can affect clinical course of the disease. Here, using primary keratinocytes we determined that atopic promotes changes in the interaction of small extracellular vesicles (sEVs) with dendritic cells and that this is further enhanced by the presence of . sEV uptake is largely dependent on the expression of glycans on their surface; modelling of the protein interactions indicated that recognition of this pathogen through -relevant pattern recognition receptors (PRRs) is linked to several glycosylation enzymes which may in turn affect the expression of sEV glycans.

Stimulation of Sulfonamides Antibacterial Drugs Activity as a Result of Complexation with Ru(III): Physicochemical and Biological Study.

Antibiotic resistance is a global problem, and one promising solution to overcome this issue is using metallodrugs, which are drugs containing metal ions and ligands. These complexes are superior to free ligands in various characteristics including anticancer properties and mechanism of action. The pharmacological potential of metallodrugs can be modulated by the appropriate selection of ligands and metal ions.

Response of physiological parameters in Dionaea muscipula J. Ellis teratomas transformed with rolB oncogene.

Background: Plant transformation with rol oncogenes derived from wild strains of Rhizobium rhizogenes is a popular biotechnology tool. Transformation effects depend on the type of rol gene, expression level, and the number of gene copies incorporated into the plant's genomic DNA. Although rol oncogenes are known as inducers of plant secondary metabolism, little is known about the physiological response of plants subjected to transformation.

Cement Paste Mixture Proportioning with Particle Packing Theory: An Ambiguous Effect of Microsilica.

Recently, the research of innovative building materials is focused on applying supplementary materials in the form of micro- and nanopowders in cementitious composites due to the growing insistence on sustainable development. Considering above, in paper, a research on the effect of microsilica and SiO nanoparticles addition to cement paste, designed with Andreasen and Andersen (AA) packing density model (PDM), in terms of its physical and mechanical properties was conducted. Density, porosity, compressive strength, hardness, and modulus of indentation were investigated and compared regarding different amount of additives used in cement paste mixes.

Potential of Silver Nanoparticles in Overcoming the Intrinsic Resistance of to Secondary Metabolites from Carnivorous Plants.

Carnivorous plants are exemplary natural sources of secondary metabolites with biological activity. However, the therapeutic antimicrobial potential of these compounds is limited due to intrinsic resistance of selected bacterial pathogens, among which represents an extreme example. The objective of the study was to overcome the intrinsic resistance of by combining silver nanoparticles (AgNPs) with secondary metabolites from selected carnivorous plant species.

The Evaluation of the Effectiveness of Reinforcement by Cemented-Carbide Plates in Two Design Variants of the Chisels Intended for Cultivation-Sowing Aggregates.

Field tribological tests of two design variants of chisels used in the teeth of a cultivation-sowing unit were carried out in this research. A characteristic feature of the first variant of chisels was the reinforcement of their contact surface and almost the entire rake surface by plates made of cemented carbides. On the other hand, the second variant of chisels was reinforced only in the area of the blade by two plates made of cemented carbides, soldered on the rake face of the elements.

Transformed tissue of Dionaea muscipula J. Ellis as a source of biologically active phenolic compounds with bactericidal properties.

The Venus flytrap (Dionaea muscipula J. Ellis) is a carnivorous plant able to synthesize large amounts of phenolic compounds, such as phenylpropanoids, flavonoids, phenolic acids, and 1,4-naphtoquinones. In this study, the first genetic transformation of D.

Impact of Impulses on Microstructural Evolution and Mechanical Performance of Al-Mg-Si Alloy Joined by Impulse Friction Stir Welding.

Impulse Friction Stir Welding (IFSW) was utilized to join 6082-T6 alloy plates at various impulse frequencies. A distinctive feature of IFSW is the generation of mechanical impulses that enhances the forging action of the tool, and thereby, alters the weld microstructure. The microstructural evolution in the Stir Zone (SZ) with special focus on the strengthening precipitation behavior, and overall mechanical properties of the IFSW joints have been investigated.

Iris pseudacorus as an easily accessible source of antibacterial and cytotoxic compounds.

Iris pseudacorus is one of the most widespread iris species and possesses complex secondary metabolites. Our study showed that its rhizomes are abundant with phenolic compounds of which 80 % belong to the tannin group. Methanolic extracts from garden cultured iris rhizomes possessed antibacterial activity against human Gram positive Staphylococcus aureus and Enterococcus faecalis and Gram negative Pseudomonas aeruginosa and Klebsiella pneumoniae pathogens including clinical isolates resistant to commercially available antibiotics.

Metallurgical Characterization of Welded Joint of Nanostructured Bainite: Regeneration Technique versus Post Welding Heat Treatment.

One of the main limitations in application of nanostructured carbide-free bainite as a construction material is the difficulty of joining. This research presents a structural characterization of welded joints of medium carbon 55Si7 grade steel after the welding process with a regeneration technique as well as post welding heat treatment (PWHT). The hardness distribution of the welded joint with regeneration exhibit an overall decrease in hardness when compared to the base material and a significant decrease in hardness was observed in the heat-affected zone (HAZ).

The Effect of the Addition of Polypropylene Fibers to Primer on the Pull-Off Strength of Epoxy Resin Coatings.

This article describes the effect of adding polypropylene fibers to primer on the pull-off strength of epoxy resin coatings. Investigated primers were laid on substrates made of cement mortar and cement slurry. The primer was made of epoxy resin modified with the addition of 0.

Antibacterial composite hybrid coatings of veterinary medical implants.

The aim of the work was to develop innovative antibacterial hybrid coatings applied on implants that are used for anastomoses of animals' long bones and to assess their physicochemical and biological properties. Plates made of the titanium alloy were covered with composite hybrid layers so as to protect the implant surface against corrosion and to enhance it with antibacterial properties.The hybrid coatings were obtained electrochemical oxidation and sol-gel.

The Influence of Microstructure on Abrasive Wear Micro-Mechanisms of the Claddings Produced by Welding Used in Agricultural Soil.

Claddings produced by welding are commonly used to increase the durability of the working elements of agricultural tools. The working conditions that occur during the cultivation of agricultural soil determine the wear intensity (different soil fractions, biological, and chemical environment). It was found that the tested claddings (Fe-Cr-C-Nb system) is characterized by three different layers: hypereutectic (layer I), near eutectic (layer II), and hypoeutectic (layer III).

Elicitation-Based Method for Increasing the Production of Antioxidant and Bactericidal Phenolic Compounds in J. Ellis Tissue.

The carnivorous plant J. Ellis (Venus flytrap) is a widely known medical herb, capable of producing various phenolic compounds known for their strong antioxidant and antibacterial properties. In the pharmaceutical industry, Venus flytrap is grown in tissue cultures, as the natural population of is very limited.

Is a blue-red light a good elicitor of phenolic compounds in the family Droseraceae? A comparative study.

Plants from the family Droseraceae, especially Drosera sp. and Dionaea sp., are naturally rich in phenolic derivatives such as plumbagin, among others.