Publications by authors named "Aleksandra Flaht-Zabost"
Histol Histopathol
January 2024
Article Synopsis
- Lymphatic vessels (LyVs) play a crucial role in maintaining fluid, solute, and immune cell balance in the body and are influenced by surrounding extracellular matrix (ECM) molecules, which affect their function and structure.
- Changes in the ECM due to disease can negatively impact the lymphatic system, leading to dysfunction in LyV networks.
- This review focuses on the current understanding of ECM molecules in various tissues, especially around lymphatic vessels in both healthy and diseased states.
Histochem Cell Biol
January 2021
Article Synopsis
- The study focuses on understanding cardiac tissue macrophages (cTMs) during fetal development, particularly their populations and functions based on specific surface markers.
- Immunostaining of murine fetal hearts revealed that cTMs are primarily located in the subepicardial space and interact with newly formed blood and lymphatic vessels.
- Three distinct subpopulations of cTMs were identified, showing different gene expression levels related to angiogenesis, lymphangiogenesis, and extracellular matrix remodeling, indicating their diverse roles in heart development.
Anat Rec (Hoboken)
June 2019
Article Synopsis
- The proepicardium (PE) is a temporary structure in embryos that is essential for heart development, originating from the lateral plate mesoderm and found in all vertebrates.
- Mesothelial cells from the PE connect to the heart's muscle layer (myocardium) to eventually form the epicardium, the heart's outer layer, through processes including epithelial-to-mesenchymal transition (EMT).
- Understanding the molecular pathways involved in PE formation and the differentiation of epicardial cells is crucial as these mechanisms may reactivate in heart diseases, highlighting their relevance for both developmental biology and potential therapeutic targets.
Histochem Cell Biol
June 2018
Article Synopsis
- Hematopoietic cells, which are crucial for blood cell formation, develop in areas where blood vessels are forming, originating from a specific type of endothelial cells called hemogenic endothelium.
- Researchers investigated whether the proepicardium, a tissue in developing embryos, contains endothelial cells with hematopoietic potential and found promising results through in vitro culture of CD31/CD45/CD71 cells.
- Their findings include the generation of various blood cell colony types and the identification of key markers indicating the presence of hematopoietic lineages, all supported by analyses showing the expression of important regulatory genes linked to hematopoietic development.
Article Synopsis
- Angiogenesis is crucial for forming blood vessels and plays a role in diseases like tumors, but the details of its molecular pathways remain unclear due to inadequate models.
- The proepicardial explant (PE) is suggested as a good model for studying angiogenesis since it contains undifferentiated endothelial cells and can produce vascular sprouts in the presence of specific growth factors like bFGF and VEGF-A.
- The research shows that these sprouts vary in characteristics based on the growth factors used and their ECs express certain mRNAs, indicating that this PE model could enhance our understanding of angiogenesis alongside existing models.
Cells Tissues Organs
May 2017
Article Synopsis
- *Recent research has revealed the role of EPCs in diseases, tumors, and healing processes, highlighting their importance in various medical conditions.
- *The overview discusses methods to activate EPCs for therapeutic use and reviews the effectiveness of these treatments.
Microvasc Res
November 2015
Article Synopsis
- The study investigates the proepicardium (PE), an embryonic structure vital for heart development, specifically focusing on its endothelial cells (ECs).
- Results show that these proepicardial ECs express certain markers that indicate they are early-stage and minimally differentiated, while forming a network connected to the sinus venosus (SV).
- The findings suggest a continuous and immature EC network in the PE, contributing to our understanding of heart embryogenesis and the role of endothelial cells in this process.
Histochem Cell Biol
February 2015
Article Synopsis
- The study investigates the phenotypic profiles and locations of potential vasculogenic and hematopoietic progenitor cells in the embryonic mouse heart by using imaging techniques and cell analysis methods.
- Researchers identified distinct groups of cells, demonstrating specific combinations of surface markers that indicate their maturity and lineage, including various combinations of GATA2, CD41, CD71, Flk1, and Fli1.
- Key findings highlight the presence of subepicardial blood islands and the functionality of endocardial wall endothelia in supporting megakaryocyte activity, underscoring a complex interplay between blood vessel formation and blood cell production in early heart development.
Anat Rec (Hoboken)
June 2014
Article Synopsis
- * Key molecular markers for cardiac lymphatic endothelial cells include Prox-1, Lyve-1, and VEGFR3, among others, differentiating them from other cell types.
- * Two main collecting vessels are identified: one drains the right and left ventricles towards lymph nodes near the trachea, while the other follows the left cardiac vein to the coronary sinus and left atrium, developing a smooth muscle layer later in postnatal life.
