Chronic administration of fluoxetine or clozapine induces oxidative stress in rat liver: a histopathological study.

Chronic exposure to stress contributes to the etiology of mood disorders, and the liver as a target organ of antidepressant and antipsychotic drug metabolism is vulnerable to drug-induced toxicity. We investigated the effects of chronic administration of fluoxetine (15mg/kg/day) or clozapine (20mg/kg/day) on liver injury via the measurement of liver enzymes, oxidative stress and histopathology in rats exposed to chronic social isolation (21days), an animal model of depression, and controls. The activity of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST), the liver content of carbonyl groups, malonyldialdehyde (MDA), reduced glutathione (GSH), cytosolic glutathione S-transferase (GST) and nitric oxide (NO) metabolites were determined.

Optimization of separation and determination of moxifloxacin and its related substances by RP-HPLC.

A RP-HPLC method for the separation and determination of impurities of moxifloxacin, in its pharmaceutical forms as well as moxifloxacin degradation products, was developed with the aid of DryLab software and chemometric (response surface) approach. The separation of four synthesis-related impurities was achieved on a Waters C(18) XTerra column using a mobile phase of (water+triethylamine (2%, v/v)): acetonitrile=90:10 (v/v%); the pH of water phase being adjusted with phosphoric acid to 6.0.

Solution equilibria between aluminum(III) ion and some fluoroquinolone family members. Spectroscopic and potentiometric study.

Complex formation between aluminum(III) ion and fluoroquinolone antibacterials-either moxifloxacin (4th generation antibiotic) or fleroxacin (2nd generation antibiotic) were studied in aqueous solutions without and in the presence of sodium dodecylsulfate (SDS). The investigations were performed by glass electrode potentiometric (ionic medium: 0.1 mol/dm(3) LiCl, 298 K), UV spectrophotometric, multinuclear (1H and 13C) magnetic resonance and ESI-MS measurements.