Coregulators Reside within Ecdysone-Inducible Loci before and after Ecdysone Treatment.

Ecdysone signaling in remains a popular model for investigating the mechanisms of steroid action in eukaryotes. The ecdysone receptor EcR can effectively bind ecdysone-response elements with or without the presence of a hormone. For years, EcR enhancers were thought to respond to ecdysone via recruiting coactivator complexes, which replace corepressors and stimulate transcription.

RNA Polymerase II "Pause" Prepares Promoters for Upcoming Transcription during Development.

According to previous studies, during embryogenesis, the recruitment of RNA polymerase II precedes active gene transcription. This work is aimed at exploring whether this mechanism is used during metamorphosis. In addition, the composition of the RNA polymerase II "paused" complexes associated with promoters at different developmental stages are described in detail.