Whole-Genome Sequencing-Based Profiling of Antimicrobial Resistance Genes and Core-Genome Multilocus Sequence Typing of from Different Sources in Lithuania.

is known as one of the main causative agents of gastroenteritis in humans worldwide, and the rise of antimicrobial resistance (AMR) in is a growing public health challenge of special concern. Whole-genome sequencing (WGS) was used to characterize genetic determinants of AMR in 53 isolates from dairy cattle, broiler products, wild birds, and humans in Lithuania. The WGS-based study revealed 26 AMR markers that conferred resistance to various antimicrobials.

Whole Genome Sequence-Based Prediction of Resistance Determinants in High-Level Multidrug-Resistant Isolates in Lithuania.

Spread of antibiotic resistance via mobile genetic elements associates with transfer of genes providing resistance against multiple antibiotics. Use of various comparative genomics analysis techniques enables to find intrinsic and acquired genes associated with phenotypic antimicrobial resistance (AMR) in genome sequences with exceptionally high-level multidrug resistance. In this study, we used whole genome sequences of seven to identify isolate-specific genomic features associated with resistance and virulence determinants and their role in multidrug resistance (MDR).

Draft Genome Sequence of Ciprofloxacin and Ceftriaxone Resistant MM26-781 Assigned to Novel ST Isolated From Common Pigeon in Lithuania.

is an important zoonotic pathogen known to be resistant to a wide range of antibiotics worldwide. may be intrinsically resistant to antibiotics or can acquire antibiotic resistance determinants through gene transfer. However, the knowledge of molecular mechanisms of antimicrobial resistance among isolates from wild birds, especially in Lithuania, is limited.

Overlap of Antibiotic Resistant MLST Genotypes Isolated From Humans, Broiler Products, Dairy Cattle and Wild Birds in Lithuania.

Antimicrobial resistance was determined for 341 thermophilic isolates obtained from human clinical cases ( = 101), broiler products ( = 98), dairy cattle ( = 41) and wild birds ( = 101) with known multilocus sequence types (MLST) in Lithuania. The minimum inhibitory concentration (MIC) values for ciprofloxacin, tetracycline, gentamicin, ceftriaxone and erythromycin were determined with the agar dilution method. MIC values were compared with MLST types to find possible associations among isolation source, sequence type and resistance to antibiotics.

Prevalence of Foodborne Pathogens in Freshwater Fish in Latvia.

The aim of this study was to detect the prevalence of Salmonella spp., Listeria monocytogenes, and Yersinia enterocolitica in freshwater fish in Latvia. In total, 235 samples, including freshly caught fish from fives lakes (n = 129) and fish from retail markets (n = 106), were collected from April 2014 to December 2014 in Latvia.

High Prevalence and Genetic Diversity of Campylobacter jejuni in Wild Crows and Pigeons.

The occurrence, seasonal variation and genetic diversity of Campylobacter spp. in pigeons and crows over a 1-year period were evaluated. Campylobacter spp.

The antimicrobial effect of spice-based marinades against Campylobacter jejuni on contaminated fresh broiler wings.

The antimicrobial effect of spice-based marinades against Campylobacter jejuni on inoculated fresh broiler wings was investigated. Experiments were carried out with 1 strain of C. jejuni and 6 marinades.

Genetic diversity and antimicrobial resistance of Yersinia enterocolitica isolated from pigs and humans in Lithuania.

Background: Yersiniosis is one of the three leading foodborne zoonoses in Lithuania, and the incidence of 12.86 per 100,000 population was the highest among EU member states in 2010. Contaminated pig carcasses and subsequently undercooked pig meat are considered to be the primary transmission vehicle of enteropathogenic Y.

Prevalence and genetic diversity of enteropathogenic Yersinia spp. in pigs at farms and slaughter in Lithuania.

The prevalence of enteropathogenic Yersinia spp. in pigs at farms and slaughter in relation to potential farming risk factors in Lithuania was examined. Pig faeces and carcase swab samples from 11 farms were studied at slaughterhouses.