Publications by authors named "Alejandro Valda"
Background And Purpose: The determination of the total metabolic tumour volume based on [F]fluorothymidine ([F]FLT) PET/CT images in diffuse large B-cell lymphoma has a potential clinical value for detecting early relapse in this type of heterogeneous lymphoproliferative tumours. Tumour segmentation is a key step in this process. For this purpose, our objective was to determine a segmentation threshold of [F]FLT PET/CT images, based on a reference tissue uptake, on a cohort of patients with diffuse large B-cell lymphoma (DLBCL) that have been scanned at different stages of the treatment.
View Article and Find Full Text PDFPositron emission tomography-computed tomography (PET-CT) is part of the new diagnostic and therapeutic algorithms for Hodgkin lymphoma. PET-CT is a valuable tool for the assessment of treatment response and prognosis, both by means of interim PET-CT with 2-[18F]fluoro-2-deoxy-D-glucose ([18F]FDG) as well as end of treatment (EOT) PET-CT. Given the low specificity of [18F]FDG for the diffuse large B cell lymphoma (DLBCL), there is an emerging need for a more specific radiopharmaceutical agent.
View Article and Find Full Text PDFAim: This work aims at giving an updated report of the worldwide status of Accelerator-Based BNCT (AB-BNCT).
Background: There is a generalized perception that the availability of accelerators installed in hospitals, as neutron sources, may be crucial for the advancement of BNCT. Accordingly, in recent years a significant effort has started to develop such machines.
Purpose: To evaluate the cell response to DNA double-strand breaks induced by low and high linear energy transfer (LET) radiations when the catalytic subunit of DNA-dependent protein kinase (DNA-PKcs), an essential protein of the nonhomologous end-joining repair pathway, lacks kinase activity.
Methods And Materials: CHO10B2, a Chinese hamster ovary cell line, and its derived radiosensitive mutant cell line, irs-20, lacking DNA-PKcs activity, were evaluated after 0 to 3 Gy of γ-rays, plateau and Bragg peak protons, and lithium beams by clonogenic assay, and as a measurement of double-strand breaks, phosphorylated H2AX (γH2AX) foci number and size were quantified by immunocytofluorescence.
Results: Irs-20 exhibited greater radiosensitivity and a higher amount of γH2AX foci than CHO10B2 at 6 hours after irradiation for all types of radiations.
Article Synopsis
- - The study aimed to assess how melanoma cells (B16-F0) respond to DNA double strand breaks (DSBs) after being exposed to various types of radiation, specifically focusing on the impact of low and high linear energy transfer (LET) radiation.
- - Researchers measured cell survival and DSBs through clonogenic assays and the detection of gammaH2AX foci at different time points after exposure, revealing that higher LET radiation led to a greater effectiveness in inducing DSBs.
- - The findings indicated that the size of gammaH2AX foci could be used as a reliable measure to understand how effectively DSBs are repaired and how sensitive the cells are to the different types of radiation, with larger foci