- The study aimed to assess how melanoma cells (B16-F0) respond to DNA double strand breaks (DSBs) after being exposed to various types of radiation, specifically focusing on the impact of low and high linear energy transfer (LET) radiation.
- Researchers measured cell survival and DSBs through clonogenic assays and the detection of gammaH2AX foci at different time points after exposure, revealing that higher LET radiation led to a greater effectiveness in inducing DSBs.
- The findings indicated that the size of gammaH2AX foci could be used as a reliable measure to understand how effectively DSBs are repaired and how sensitive the cells are to the different types of radiation, with larger foci