Publications by authors named "Alejandra Isabel"
Discordant results between genotypic and phenotypic assays (Xpert MTB/RIF vs. BACTEC MGIT 960 system) for detection of RIF-resistant Mycobacterium tuberculosis isolates in a high burden region.
Laniado-Laborín Rafael Muñiz-Salazar Raquel Flores-Acosta Rogelio Félix-Ponce Miroslava Jiménez-Gracia Alejandra-Isabel
Infect Genet Evol
December 2021
Article Synopsis
- A study was conducted on clinical isolates with conflicting results from phenotypic and genotypic tests for rifampin resistance in TB at the Tijuana General Hospital in Mexico.
- Out of 13 isolates tested, discrepancies were found where some were classified as rifampin-susceptible yet resistant according to another test, with DNA sequencing revealing various mutations in some isolates.
- The findings highlighted the importance of further DNA sequencing for accurate diagnoses of rifampin resistance, especially due to cases of heteroresistance and silent mutations.
The CII protein of temperate coliphage 186, like the unrelated CII protein of phage λ, is a transcriptional activator that primes expression of the CI immunity repressor and is critical for efficient establishment of lysogeny. 186-CII is also highly unstable, and we show that in vivo degradation is mediated by both FtsH and RseP. We investigated the role of CII instability by constructing a 186 phage encoding a protease resistant CII.
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