Publications by authors named "Alejandra Coronado"
J Microbiol Biotechnol
May 2013
Article Synopsis
- The study focuses on optimizing the production of the enzyme N-acetylgalactosamine-6-sulfate sulfatase (GALNS) using E. coli by examining various factors like signal peptides, inducer concentrations, and operational modes.
- The presence of a native signal peptide increased enzyme activity, and its removal negatively affected enzyme activation.
- Optimal IPTG concentrations were identified for both shake and bench scales, with greater enzyme activity and secretion occurring in semi-continuous modes when the signal peptide was present, highlighting implications for producing human sulfatases in E. coli.
